Anti-CSPG4 fusions with interferon for the treatment of malignancy

ABSTRACT

In various embodiments chimeric moieties (constructs) are provided that show significant efficacy against cancers. In certain embodiments the constructs comprise a targeting moiety that specifically binds CSPG4 attached to an interferon or to a mutant interferon. In certain embodiments, the constructs comprise anti-CSPG4 antibody attached to an interferon alpha (IFN-α) or to a mutant interferon alpha or to an interferon beta (IFN-β) or to a mutant interferon beta, or to an interferon gamma (IFN-γ) or to a mutant interferon gamma.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a U.S. 371 National Phase of PCT/US2014/040036, filed on May 29, 2014, which claims benefit of and priority to U.S. Ser. No. 61/828,590, filed on May 29, 2013, all of which are incorporated herein by reference in their entirety for all purposes.

STATEMENT OF GOVERNMENTAL SUPPORT

[Not Applicable]

BACKGROUND

Malignant melanoma is an immunogenic, highly aggressive and often lethal form of skin cancer. It is the most common cancer in the 17-34 years age group but affects people of all ages, and therefore has a significant socioeconomic impact for patients and their families. Although diagnosed skin lesions can be initially excised by surgical intervention, skin and distal metastases unfortunately occur in 20% of patients originally treated with local disease. Patients with lymph node and other distal metastases have dismal prognosis, and this is partly due to lack of effective treatments for this cohort.

Melanoma has presented major challenges to numerous targeted therapy efforts and therefore effective treatments are urgently needed for patients with this disease. The recent approval of the monoclonal antibody ipilimumab (targeting the CTLA4 blockade to enhance T cell activation) for the treatment of melanoma lends merit to the notion that activating immune responses with antibodies may have therapeutic significance and has renewed interest in the field of antibody therapies for the treatment of challenging tumours such as melanoma.

Interferons have been contemplated for use in the treatment of cancer (Borden et al. (2005) J. Interferon Cytokine Res. 25: 511-527; Borden et al. (2007) Nat. Rev. Drug Discov. 6: 975-690). There are seven classes of type I IFNs with IFNα and IFNβ being the most abundant. Both IFNα and IFNβ bind to the same receptor composed of two transmembrane proteins, IFNAR 1 and 2, but IFNβ binds with much higher affinity than IFNα (Lamken et al. (2004) J. Mol. Biol. 341: 303-318). IFNs have been shown to have anti-proliferative activity as well as the ability to induce apoptosis in hematological malignancies and solid tumors in addition to their anti-viral activity (as reviewed in Borden et al. (2007) Nat. Rev. Drug Discov. 6: 975-690). However, the effectiveness of IFNα for cancer therapy is overshadowed by side effects when used at high doses (Weiss (1998) Semin. Oncol. 25: 9-13) and by a short half-life, e.g., of only 1 hour (Peleg-Shulman et al. (2004) J. Med. Chem. 47: 4897-4904). Strategies to increase the half-life have included the covalent linkage of polyethylene glycols (PEG) to IFNα (Talpaz et al. (2001) Blood, 98: 1708-1713), but such modifications have resulted in lower activity (Rosendahl et al. (2005) Bioconjug. Chem. 16: 200-207).

SUMMARY

In various embodiments this invention pertains to the discovery that attaching an interferon to a targeting moiety (e.g., a molecule that specifically and/or preferentially binds a marker on or associated with a cell) substantially improves the therapeutic efficacy of the interferon and appears to reduce systemic toxicity. Accordingly, in various embodiments, this invention provides constructs comprising an interferon attached to a targeting moiety and uses of such constructs to specifically and/or preferentially inhibit the growth or proliferation or even to kill certain target cells (e.g., cancer cells). In certain embodiments the constructs comprise a mutant interferon, e.g., a mutant IFNα with higher affinity for the IFNAR to enhance the potency of the construct.

Accordingly, in certain embodiments, a chimeric construct is provided where the construct comprises an interferon (e.g., interferon-alpha, interferon-beta, interferon-gamma, mutant interferon-α, mutant interferon-β, and the like) attached to a targeting moiety that binds to a tumor associated antigen, in particular CSPG4. The construct when contacted to a tumor cell results in the killing or inhibition of growth or proliferation of the tumor cell.

In various aspects, the invention(s) contemplated herein may include, but need not be limited to, any one or more of the following embodiments

Embodiment 1

A chimeric construct including an interferon attached to an antibody that binds chondroitin sulfate proteoglycan 4 (CSPG4).

Embodiment 2

The construct of embodiment 1, wherein said construct when contacted to a cell that expresses or overexpresses CSPG4 cell results in the killing or inhibition of growth or proliferation of said cell.

Embodiment 3

The construct of embodiment 2, wherein said cell that expresses or overexpresses CSPG4 is a cancer cell.

Embodiment 4

The construct of embodiment 2, wherein said cell that expresses or overexpresses CSPG4 is a cancer selected from the group consisting of tumors of neuroectodermal origin including melanoma and glioma, breast cancer including triple negative breast cancer, squamonous cell carcinoma of head and neck, myeloid leukemia, pancreatic carcinoma, chondrosarcoma, chordoma, mesothelioma, renal cell carcinoma, lung carcinoma, ovarian carcinoma and cancer stem cells representing various histologies.

Embodiment 5

The construct of embodiment 2, wherein said cell that expresses or overexpresses CSPG4 is a cancer stem cell.

Embodiment 6

The construct according to any one of embodiments 1-5, wherein said interferon is a type I interferon.

Embodiment 7

The construct of embodiment 6, wherein said interferon is an interferon-alpha (IFNα).

Embodiment 8

The construct of embodiment 6, wherein said interferon is an IFN-α2.

Embodiment 9

The construct of embodiment 6, wherein said interferon is an IFN-α10.

Embodiment 10

The construct of embodiment 6, wherein said interferon is an IFN-α114.

Embodiment 11

The construct of embodiment 6, wherein said interferon is an interferon-beta (IFNβ).

Embodiment 12

The construct according to any one of embodiments 1-5, wherein said interferon is a type II interferon (IFNγ).

Embodiment 13

The construct of embodiment 12, wherein said interferon gamma is a full-length interferon gamma.

Embodiment 14

The construct of embodiment 12, wherein said interferon gamma is a truncated interferon gamma.

Embodiment 15

The construct of embodiment 12, wherein said interferon gamma is an interferon gamma having 1-15 amino acids truncated from the carboxyl terminus and/or 1-3 amino acids truncated from the amino terminus.

Embodiment 16

The construct of embodiment 12, wherein said interferon gamma is a truncated interferon gamma where the amino acid sequence of said truncated interferon gamma consists of the sequence DPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQM (SEQ ID NO:29).

Embodiment 17

The construct according to any one of embodiments 1-16, wherein said interferon is a human interferon.

Embodiment 18

The construct according to any one of embodiments 1-16, wherein said interferon is a non-human interferon.

Embodiment 19

The construct of embodiment 18, wherein said interferon is a murine interferon.

Embodiment 20

The construct according to any one of embodiments 1-5, wherein said interferon is a mutant interferon gamma.

Embodiment 21

The construct according to any one of embodiments 1-5, wherein said interferon is a mutant type I interferon.

Embodiment 22

The construct of embodiment 21, wherein said interferon is a mutant interferon-alpha.

Embodiment 23

The construct of embodiment 21, wherein said interferon is a mutant interferon-alpha having lower activity than native interferon alpha.

Embodiment 24

The construct of embodiment 21, wherein said interferon is a mutant interferon-alpha having higher activity than native interferon alpha.

Embodiment 25

The construct of embodiment 21, wherein said interferon is a mutant human interferonα-2 having mutations at one or more sites selected from the group consisting of His57, Glu58, and Gln61.

Embodiment 26

The construct of embodiment 25, wherein said interferon is an interferonα-2 having a mutation at His57.

Embodiment 27

The construct of embodiment 26, wherein said mutation at His57 is a mutation to an amino acid selected from the group consisting of A, Y, and M.

Embodiment 28

The construct according to any one of embodiments 25-27, wherein said interferon is an interferon α-2 having a mutation at Glu58.

Embodiment 29

The construct of embodiment 28, wherein said mutation at Glu58 is a mutation to an amino acid selected from the group consisting of A, N, D, and L.

Embodiment 30

The construct according to any one of embodiments 25-29, wherein said interferon is an interferonα-2 having a mutation at Gln61.

Embodiment 31

The construct of embodiment 30, wherein said mutation at Gln61 is a mutation to an amino acid selected from the group consisting of A, S, and D.

Embodiment 32

The construct of embodiment 25, wherein said interferon includes the mutations H57Y, E58N, and Q61S.

Embodiment 33

The construct of embodiment 25, wherein said interferon includes the mutations H57M, E58L, and Q61D.

Embodiment 34

The construct of embodiment 25, wherein said interferon includes the mutations H57Y, E58L, and Q61D.

Embodiment 35

The construct of embodiment 25, wherein said interferon includes the mutations H57Y, E58A, and Q61S.

Embodiment 36

The construct of embodiment 25, wherein said interferon includes the mutations H57A, E58A, and Q61A.

Embodiment 37

The construct according to any one of embodiments 1-36, wherein said antibody binds to a CSPG4 at an epitope bound by one or more antibodies selected from the group consisting of 9.2.27, VF1-TP34, VF1-TP34, VF1-TP41.2, TP61.5, 149.53, 149.53, 225.28, 225.28s, 763.74, and scFv-FcC21.

Embodiment 38

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of an antibody selected from the group consisting of 9.2.27, VF1-TP34, VF1-TP34, VF1-TP41.2, TP61.5, 149.53, 149.53, 225.28, 225.28s, 763.74, and scFv-FcC21.

Embodiment 39

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the 92.2.27 antibody.

Embodiment 40

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the 225.28 antibody.

Embodiment 41

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the scFv-FcC21 antibody.

Embodiment 42

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the VF1-TP34 antibody.

Embodiment 43

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the VF1-TP34 antibody.

Embodiment 44

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the VF1-TP41.2 antibody.

Embodiment 45

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the TP61.5 antibody.

Embodiment 46

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the 149.53 antibody.

Embodiment 47

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the 149.53 antibody.

Embodiment 48

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the 225.28s antibody.

Embodiment 49

The construct of embodiment 37, wherein said antibody includes at least 3 complementarity determining regions from the VH domain of the 763.74 antibody.

Embodiment 50

The construct according to any one of embodiments 37-49, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of an antibody selected from the group consisting of VF1-TP34, VF1-TP34, VF1-TP41.2, TP61.5, 9.2.27, 149.53, 149.53, 225.28, 225.28s, 763.74, and scFv-FcC21.

Embodiment 51

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the 92.2.27 antibody.

Embodiment 52

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the 225.28 antibody.

Embodiment 53

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the scFv-FcC21 antibody.

Embodiment 54

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the VF1-TP34 antibody.

Embodiment 55

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the VF1-TP34 antibody.

Embodiment 56

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the VF1-TP41.2 antibody.

Embodiment 57

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the TP61.5 antibody.

Embodiment 58

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the 149.53 antibody.

Embodiment 59

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the 149.53 antibody.

Embodiment 60

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the 225.28s antibody.

Embodiment 61

The construct of embodiment 50, wherein said antibody includes at least 3 complementarity determining regions from the VL domain of the 763.74 antibody.

Embodiment 62

The construct of embodiment 37, wherein said antibody includes the VH and/or VL domain of an antibody selected from the group consisting of VF1-TP34, VF1-TP34, VF1-TP41.2, TP61.5, 9.2.27, 149.53, 149.53, 225.28, 225.28s, 763.74, and scFv-FcC21.

Embodiment 63

The construct of embodiment 62, wherein said antibody includes the VH and the VL domain of an antibody selected from the group consisting of VF1-TP34, VF1-TP34, VF1-TP41.2, TP61.5, 9.2.27, 149.53, 149.53, 225.28, 225.28s, 763.74, and scFv-FcC21.

Embodiment 64

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the 92.2.27 antibody.

Embodiment 65

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the 225.28 antibody.

Embodiment 66

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the scFv-FcC21 antibody.

Embodiment 67

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the VF1-TP34 antibody.

Embodiment 68

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the VF1-TP34 antibody.

Embodiment 69

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the VF1-TP41.2 antibody.

Embodiment 70

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the TP61.5 antibody.

Embodiment 71

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the 149.53 antibody.

Embodiment 72

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the 149.53 antibody.

Embodiment 73

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the 225.28s antibody.

Embodiment 74

The construct of embodiment 63, wherein said antibody includes the VH and the VL domain of the 763.74 antibody.

Embodiment 75

The construct of embodiment 37, wherein said antibody is the 9.2.27 antibody.

Embodiment 76

The construct of embodiment 37, wherein said antibody is the VF1-TP34 antibody.

Embodiment 77

The construct of embodiment 37, wherein said antibody is the VF1-TP41.2 antibody.

Embodiment 78

The construct of embodiment 37, wherein said antibody is the TP61.5 antibody.

Embodiment 79

The construct of embodiment 37, wherein said antibody is the 149.53 antibody.

Embodiment 80

The construct of embodiment 37, wherein said antibody is the 225.28 antibody.

Embodiment 81

The construct of embodiment 37, wherein said antibody is the 225.28s antibody.

Embodiment 82

The construct of embodiment 37, wherein said antibody is the 763.74 antibody.

Embodiment 83

The construct of embodiment 37, wherein said antibody is the scFv-FcC21 antibody.

Embodiment 84

The construct according to any one of embodiments 1-74, wherein said antibody is an antibody selected from the group consisting of a single chain Fv (scFv), a FAB, a (Fab′)₂, an (scFv)₂, and a full immunoglobulin.

Embodiment 85

The construct of embodiment 84, wherein said antibody is an scFv.

Embodiment 86

The construct of embodiment 84, wherein said antibody is a full immunoglobulin.

Embodiment 87

The construct of embodiment 86, wherein said antibody is an IgE.

Embodiment 88

The construct of embodiment 86, wherein said antibody is an IgG.

Embodiment 89

The construct of embodiment 86, wherein said antibody is an IgA.

Embodiment 90

The construct of embodiment 86, wherein said antibody is an IgM.

Embodiment 91

The construct of embodiment 86, wherein said antibody is an IgD.

Embodiment 92

The construct according to any of embodiments 1-91, wherein said antibody is chemically coupled to said interferon.

Embodiment 93

The construct according to any of embodiments 1-91, wherein said antibody is directly joined to said interferon.

Embodiment 94

The construct according to any of embodiments 1-91, wherein said antibody is joined to said interferon with a peptide linker.

Embodiment 95

The construct of embodiment 94, wherein said peptide linker joins said interferon to the carboxyl terminus of the CH3 domain of said antibody.

Embodiment 96

The construct of embodiment 95, wherein said peptide linker joins the amino terminus of said interferon to the carboxyl terminus of the CH3 domain of said antibody.

Embodiment 97

The construct of embodiment 95, wherein said peptide linker joins the carboxyl terminus of said interferon to the carboxyl terminus of the CH3 domain of said antibody.

Embodiment 98

The construct according to any one of embodiments 94-97, wherein said peptide linker is proteolysis resistant.

Embodiment 99

The construct according to any one of embodiments 94-98, wherein said peptide linker is fewer than 15 amino acids in length.

Embodiment 100

The construct according to any one of embodiments 94-99, wherein said peptide linker is not (Gly₄Ser)₃.

Embodiment 101

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is selected from the group consisting of GGG, GGS, GGGGS (SEQ ID NO:31), SGGGGS (SEQ ID NO:32), GGGGSGGGGS (SEQ ID NO:33), A EAAAK A (SEQ ID NO:34), A EAAAK EAAAK A (SEQ ID NO:35), A EAAAK EAAAK EAAAK A (SEQ ID NO:36), A EAAAK EAAAK EAAAK EAAAK A (SEQ ID NO:37), A EAAAK EAAAK EAAAK EAAAK EAAAK A (SEQ ID NO:38), AEAAAKEAAAKAG (SEQ ID NO:39), AEAAAKEAAAKAGS (SEQ ID NO:40), GGGGG (SEQ ID NO:41), GGAGG (SEQ ID NO:42), GGGGGGGG (SEQ ID NO:43), GAGAGAGAGA (SEQ ID NO:44), RPLSYRPPFPFGFPSVRP (SEQ ID NO:45), YPRSIYIRRRHPSPSLTT (SEQ ID NO:46), TPSHLSHILPSFGLPTFN (SEQ ID NO:47), RPVSPFTFPRLSNSWLPA (SEQ ID NO:48), SPAAHFPRSIPRPGPIRT (SEQ ID NO:49), APGPSAPSHRSLPSRAFG (SEQ ID NO:50), PRNSIHFLHPLLVAPLGA (SEQ ID NO:51), MPSLSGVLQVRYLSPPDL (SEQ ID NO:52), SPQYPSPLTLTLPPHPSL (SEQ ID NO:53), NPSLNPPSYLHRAPSRIS (SEQ ID NO:54), LPWRTSLLPSLPLRRRP (SEQ ID NO:55), PPLFAKGPVGLLSRSFPP (SEQ ID NO:56), VPPAPVVSLRSAHARPPY (SEQ ID NO:57), LRPTPPRVRSYTCCPTP (SEQ ID NO:58), PNVAHVLPLL TVPWDNLR (SEQ ID NO:59), CNPLLPLCARSPAVRTFP (SEQ ID NO:60), LGTPTPTPTPTGEF (SEQ ID NO:61), EDFTRGKL (SEQ ID NO:62), L EAAAR EAAAR EAAAR EAAAR (SEQ ID NO:63), L EAAAR EAAAR EAAAR (SEQ ID NO:64), L EAAAR EAAAR (SEQ ID NO:65), L EAAAR (SEQ ID NO:66), EAAAR EAAAR EAAAR EAAAR (SEQ ID NO:67), EAAAR EAAAR EAAAR (SEQ ID NO:68), EAAAR EAAAR (SEQ ID NO:69), EAAAR (SEQ ID NO:70), LTEEQQEGGG (SEQ ID NO:71), TEEQQEGGG (SEQ ID NO:72), LAKLKQKTEQLQDRIAGGG (SEQ ID NO:73), LELKTPLGDT THTCPRCPEP KSCDTPPPCP RCPEPKSCDT PPPCPRCPEP KSCDTPPPCP RCPGG (SEQ ID NO:74), and LEPKSSDKTHTSPPSPGG (SEQ ID NO:75).

Embodiment 102

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is selected from the group consisting of GGGGS, SGGGGS, AEAAAKEAAAKAG, and AEAAAKEAAAKAGS.

Embodiment 103

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is SGGGGS.

Embodiment 104

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is LTEEQQEGGG (SEQ ID NO:69)

Embodiment 105

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is TEEQQEGGG (SEQ ID NO:70).

Embodiment 106

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is LAKLKQKTEQLQDRIAGGG (SEQ ID NO:71).

Embodiment 107

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is LELKTPLGDT THTCPRCPEP KSCDTPPPCP RCPEPKSCDT PPPCPRCPEP KSCDTPPPCP RCPGG (SEQ ID NO:72).

Embodiment 108

The construct according to any one of embodiments 94-97, wherein the amino acid sequence of said peptide linker is LEPKSSDKTHTSPPSPGG (SEQ ID NO:73).

Embodiment 109

The construct of embodiment 1, wherein said construct includes interferon alpha attached to the 92.2.27 monoclonal antibody by a linker where the amino acid sequence of said linker includes or consists of the sequence SGGGGS.

Embodiment 110

The construct of embodiment 109, wherein said interferon is attached to the end of CH3 by said linker.

Embodiment 111

The according to any one of embodiments 1-110, wherein said construct or a component thereof is a recombinantly expressed fusion protein.

Embodiment 112

A pharmaceutical formulation including a construct according to any of embodiments 1-111 in a pharmaceutically acceptable excipient.

Embodiment 113

The pharmaceutical formulation according to embodiment 112, wherein said formulation is a unit dosage formulation.

Embodiment 114

The pharmaceutical formulation according to embodiment 112, wherein said formulation is a formulated for parenteral administration.

Embodiment 115

The pharmaceutical formulation according to embodiment 112, wherein said formulation is a formulated for administration via a route selected from the group consisting of oral administration, intravenous administration, intramuscular administration, direct tumor administration, inhalation, rectal administration, vaginal administration, transdermal administration, and subcutaneous depot administration.

Embodiment 116

A method of inhibiting growth and/or proliferation of a cell that expresses or overexpresses CSPG4, said method including contacting said cell with a chimeric construct according to any of embodiments 1-111, or a formulation according to any one of embodiments 112-115 in an amount sufficient to inhibit growth or proliferation of said cell.

Embodiment 117

The method of embodiment 116, wherein said cell is a cancer cell.

Embodiment 118

The method of embodiment 117, wherein said cancer cell is a metastatic cell.

Embodiment 119

The method of embodiment 117, wherein said cancer cell is in a solid tumor.

Embodiment 120

The method of embodiment 117, wherein said cancer cell is cell produced by a cancer selected from the group consisting of tumors of neuroectodermal origin including melanoma and glioma, breast cancer including triple negative breast cancer, squamonous cell carcinoma of head and neck, myeloid leukemia, pancreatic carcinoma, chondrosarcoma, chordoma, mesothelioma, renal cell carcinoma, lung carcinoma, ovarian carcinoma and cancer stem cells representing various histologiesmelanoma, breast cancer, and glioma.

Embodiment 121

The method of embodiment 117, wherein said cancer cell is a metastatic cell.

Embodiment 122

The method of embodiment 117, wherein said cancer cell is a cell of a metastatic melanoma.

Embodiment 123

The method according to any one of embodiments 116-122, wherein said method includes inhibiting, delaying and/or preventing the growth of a tumor and/or spread of malignant tumor cells.

Embodiment 124

The method according to any one of embodiments 116-123, wherein said contacting includes systemically administering said construct or formulation to a mammal.

Embodiment 125

The method according to any one of embodiments 116-123, wherein said contacting includes administering said construct or formulation directly into a tumor site.

Embodiment 126

The method according to any one of embodiments 116-123, wherein said contacting includes administering said construct or formulation via a route selected from the group consisting of oral administration, intravenous administration, intramuscular administration, direct tumor administration, inhalation, rectal administration, vaginal administration, transdermal administration, and subcutaneous depot administration.

Embodiment 127

The method according to any one of embodiments 116-123, wherein said contacting includes administering said construct or formulation intravenously.

Embodiment 128

The method according to any one of embodiments 116-127, wherein said cell is a cell in a human.

Embodiment 129

The method according to any one of embodiments 116-127, wherein said cell is a cell in a non-human mammal.

Embodiment 130

The method of embodiment 116, wherein said contacting includes systemically administering said construct or formulation to a mammal.

Embodiment 131

The method of embodiment 116, wherein said contacting includes administering said construct or formulation directly into a tumor site.

Embodiment 132

The method of embodiment 116, wherein said contacting includes intravenous administration of said construct or formulation.

Embodiment 133

The method of embodiment 116, wherein said cancer cell is a cancer cell in a human.

Embodiment 134

The method of embodiment 116, wherein said cancer cell is a cancer cell in a non-human mammal.

Embodiment 135

The method according to any one of embodiments 117-134, wherein said method further includes administering to said subject one or more cytotoxic agents and/or radiation in an amount effective to reduce tumor load, wherein said construct or formulation inhibits, delays or prevents the growth and/or spread of tumor cells including CSPG4 expressing cells.

Embodiment 136

A method for inhibiting, delaying and/or preventing the growth of a tumor and/or spread of malignant tumor cells in a subject in need thereof, said method including: administering to said subject a chimeric construct according to any of embodiments 1-111, or a formulation according to any one of embodiments 112-115; and administering to said subject one or more cytotoxic agents and/or radiation in an amount effective to reduce tumor load, wherein said immunoconjugate inhibits, delays or prevents the growth and/or spread of tumor cells including CSPG4 expressing cells.

Embodiment 137

A nucleic acid that encodes a fusion protein, said fusion protein including an interferon attached to an anti-CSPG4 single-chain antibody or to a polypeptide including an anti-CSPG4 chain antibody.

Embodiment 138

The nucleic acid of embodiment 137, wherein said interferon is an interferon as found in a construct according to any of embodiments 1-111.

Embodiment 139

The nucleic acid according to any one of embodiments 137-138, wherein said antibody is an anti-CSPG4 antibody as found in a construct according to any of embodiments 1-111.

Embodiment 140

The nucleic acid according to any one of embodiments 137-139, wherein said nucleic acid encodes a construct or a component of a construct according to any of embodiments 1-111.

Embodiment 141

A cell including a nucleic acid that expresses a fusion protein, said cell including a nucleic acid according to any of embodiments 137-140.

DEFINITIONS

The terms “polypeptide”, “peptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical analogue of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers. The term also includes variants on the traditional peptide linkage joining the amino acids making up the polypeptide. Preferred “peptides”, “polypeptides”, and “proteins” are chains of amino acids whose alpha carbons are linked through peptide bonds. The terminal amino acid at one end of the chain (amino terminal) therefore has a free amino group, while the terminal amino acid at the other end of the chain (carboxy terminal) has a free carboxyl group. As used herein, the term “amino terminus” (abbreviated N-terminus) refers to the free α-amino group on an amino acid at the amino terminal of a peptide or to the α-amino group (imino group when participating in a peptide bond) of an amino acid at any other location within the peptide. Similarly, the term “carboxy terminus” refers to the free carboxyl group on the carboxy terminus of a peptide or the carboxyl group of an amino acid at any other location within the peptide. Peptides also include essentially any polyamino acid including, but not limited to peptide mimetics such as amino acids joined by an ether as opposed to an amide bond.

An “antibody”, as used herein, refers to a protein consisting of one or more polypeptides substantially encoded by immunoglobulin genes or fragments of immunoglobulin genes. In certain embodiments, the immunoglobulin genes are human immunoglobulin genes. Recognized immunoglobulin genes include the kappa, lambda, alpha, gamma, delta, epsilon and mu constant region genes, as well as myriad immunoglobulin variable region genes. Light chains are typically classified as either kappa or lambda. Heavy chains are typically classified as gamma, mu, alpha, delta, or epsilon, which in turn define the immunoglobulin classes, IgG, IgM, IgA, IgD and IgE, respectively.

A typical (native) immunoglobulin (antibody) structural unit comprises a tetramer. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one “light” (about 25 kD) and one “heavy” chain (about 50-70 kD). The N-terminus of each chain defines a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The terms variable light chain (V_(L)) and variable heavy chain (V_(H)) refer to these regions of the light and heavy chains respectively. It is noted that immunoglobulins IgA and IgM contain multiple copies of the four chain structure.

Antibodies exist as intact immunoglobulins (also referred to as a “full antibody” or a “full-length antibody”) or as a number of well characterized fragments produced by digestion with various peptidases or expressed de novo. Thus, for example, pepsin digests an antibody below the disulfide linkages in the hinge region to produce F(ab)′₂, a dimer of Fab which itself is a light chain joined to V_(H)-C_(H)1 by a disulfide bond. The F(ab)′₂ may be reduced under mild conditions to break the disulfide linkage in the hinge region thereby converting the (Fab′)₂ dimer into an Fab′ monomer. The Fab′ monomer is essentially an Fab with part of the hinge region (see, Fundamental Immunology, W. E. Paul, ed., Raven Press, N.Y. (1993), for a more detailed description of other antibody fragments). While various antibody fragments are defined in terms of the digestion of an intact antibody, one of skill will appreciate that such Fab′ fragments may be synthesized de novo either chemically or by utilizing recombinant DNA methodology. Thus, the term antibody, as used herein also includes antibody fragments either produced by the modification of whole antibodies or synthesized de novo using recombinant DNA methodologies, including, but are not limited to, Fab′₂, IgG, IgM, IgA, IgE, scFv, dAb, nanobodies, unibodies, and diabodies. In various embodiments preferred antibodies include, but are not limited to Fab′₂, IgG, IgM, IgA, IgE, and single chain antibodies, more preferably single chain Fv (scFv) antibodies in which a variable heavy and a variable light chain are joined together (directly or through a peptide linker) to form a continuous polypeptide.

In certain embodiments antibodies and fragments used in the constructs described herein can be bispecific. Bispecific antibodies or fragments can be of several configurations. For example, bispecific antibodies may resemble single antibodies (or antibody fragments) but have two different antigen binding sites (variable regions). In various embodiments bispecific antibodies can be produced by chemical techniques (Kranz et al. (1981) Proc. Natl. Acad. Sci. USA, 78: 5807), by “polydoma” techniques (see, e.g., U.S. Pat. No. 4,474,893), or by recombinant DNA techniques. In certain embodiments bispecific antibodies of the present invention can have binding specificities for at least two different epitopes at least one of which is a tumor associate antigen. In various embodiments the antibodies and fragments can also be heteroantibodies. Heteroantibodies are two or more antibodies, or antibody binding fragments (e.g., Fab) linked together, each antibody or fragment having a different specificity.

An “antigen-binding site” or “binding portion” refers to the part of an immunoglobulin molecule that participates in antigen binding. The antigen binding site is formed by amino acid residues of the N-terminal variable (“V”) regions of the heavy (“H”) and light (“L”) chains. Three highly divergent stretches within the V regions of the heavy and light chains are referred to as “hypervariable regions” which are interposed between more conserved flanking stretches known as “framework regions” or “FRs”. Thus, the term “FR” refers to amino acid sequences that are naturally found between and adjacent to hypervariable regions in immunoglobulins. In an antibody molecule, the three hypervariable regions of a light chain and the three hypervariable regions of a heavy chain are disposed relative to each other in three dimensional space to form an antigen binding “surface”. This surface mediates recognition and binding of the target antigen. The three hypervariable regions of each of the heavy and light chains are referred to as “complementarity determining regions” or “CDRs” and are characterized, for example by Kabat et al. Sequences of proteins of immunological interest, 4th ed. U.S. Dept. Health and Human Services, Public Health Services, Bethesda, Md. (1987).

The term “interferon” refers to a full-length interferon or to an interferon fragment (truncated interferon) or interferon mutant, that substantially retains the biological activity of the full length wild-type interferon (e.g., retains at least 50%, or preferably at least 60%, or preferably at least 70%, or preferably at least 80%, preferably at least 90%, more preferably at least 95%, 98%, or 99% of the full-length interferon in its free form (e.g., when not a component of a chimeric construct). Interferons include type I interferons (e.g., interferon-alpha and interferon-beta) as well as type II interferons (e.g., interferon-gamma). The interferon (e.g., IFN-α) can be from essentially any mammalian species. In certain preferred embodiments, the interferon is from a species selected from the group consisting of human, equine, bovine, rodent, porcine, lagomorph, feline, canine, murine, caprine, ovine, a non-human primate, and the like. In various embodiments the mutated interferon comprises one or more amino acid substitutions, insertions, and/or deletions.

A single chain Fv (“sFv” or “scFv”) polypeptide is a covalently linked V_(H):V_(L) heterodimer which, in certain embodiments, may be expressed from a nucleic acid including V_(H)- and V_(L)-encoding sequences either joined directly or joined by a peptide-encoding linker. Huston et al. (1998) Proc. Nat. Acad. Sci. USA, 85: 5879-5883. A number of approaches for converting the naturally aggregated, but chemically separated light and heavy polypeptide chains from an antibody V region into an sFv molecule that will fold into a three dimensional structure substantially similar to the structure of an antigen-binding site are known (see, e.g., U.S. Pat. Nos. 5,091,513 and 5,132,405, and 4,956,778).

Chrondroitin sulfate proteoglycan 4 (CSPG4) consisting of a protein core and a chondroitin sulfate side chain is also known as high-molecular weight melanoma associated antigen (HMW-MAA) and melanoma chondroitin sulface proteoglycan (MCSP). It has been studied as a target for the treatment of melanoma. This tumor antigen is highly expressed on greater than 80% of human melanomas and has a restricted distribution in normal tissues. CSPG4 plays an important role in the biology of melanoma cells through its modulation of integrin function and enhanced growth factor receptor-regulated pathways including sustained activation of ERK 1,2. It is also expressed on cancer-initiating cells and a broad range of other tumors including breast cancer including triple negative breast cancer, glioma, squamonous cell carcinoma of head and neck, myeloid leukemic cells, pancreatic carcinoma, chondrosarcoma, chordoma, mesothelioma, renal cell carcinoma, lung carcinoma, cancer stem cells, and ovarian carcinoma. Expression of CSPG4 is associated with the progression of many different cancers.

The phrase “inhibition of growth and/or proliferation” of a cancer cell refers to decrease in the growth rate and/or proliferation rate of a cancer cell. In certain embodiments this includes death of a cancer cell (e.g. via apoptosis). In certain embodiments this term also refers to inhibiting the growth and/or proliferation of a solid tumor and/or inducing tumor size reduction or elimination of the tumor.

The term “cancer marker” refers to biomolecules such as proteins, carbohydrates, glycoproteins, and the like that are exclusively or preferentially or differentially expressed on a cancer cell and/or are found in association with a cancer cell and thereby provide targets preferential or specific to the cancer. In various embodiments the preferential expression can be preferential expression as compared to any other cell in the organism, or preferential expression within a particular area of the organism (e.g. within a particular organ or tissue).

The terms “subject,” “individual,” and “patient” may be used interchangeably and refer to a mammal, preferably a human or a non-human primate, but also domesticated mammals (e.g., canine or feline), laboratory mammals (e.g., mouse, rat, rabbit, hamster, guinea pig), and agricultural mammals (e.g., equine, bovine, porcine, ovine). In various embodiments, the subject can be a human (e.g., adult male, adult female, adolescent male, adolescent female, male child, female child) under the care of a physician or other health worker in a hospital, psychiatric care facility, as an outpatient, or other clinical context. In certain embodiments, the subject may not be under the care or prescription of a physician or other health worker.

The phrase “cause to be administered” refers to the actions taken by a medical professional (e.g., a physician), or a person controlling medical care of a subject, that control and/or permit the administration of the agent(s)/compound(s) at issue to the subject. Causing to be administered can involve diagnosis and/or determination of an appropriate therapeutic or prophylactic regimen, and/or prescribing particular agent(s)/compounds for a subject. Such prescribing can include, for example, drafting a prescription form, annotating a medical record, and the like. Where administration is described herein, “causing to be administered” is also contemplated.

The term “exhibiting IFN gamma activity” is intended to indicate that the polypeptide has one or more of the functions of native IFNγ, in particular huIFNγ or rhuIFNγ. Such functions include, inter alia, the capability to bind to an IFNγ receptor and cause transduction of the signal transduced upon huIFNγ-binding of its receptor as determined in vitro or in vivo (i.e., in vitro or in vivo bioactivity). The IFNγ receptor has been described by Aguet et al. (1988) Cell 55: 273-280) and Calderon et al. (1988) Proc. Natl. Acad. Sci. USA, 85:4837-4841. The “IFNγ polypeptide” is a polypeptide exhibiting IFNγ activity, and is used herein about the polypeptide in monomer or dimeric form, as appropriate. For instance, when specific substitutions are indicated these are normally indicated relative to the IFNγ polypeptide monomer. When reference is made to the IFNγ as part of a conjugate this is normally in dimeric form (and thus, e.g., comprises two IFNγ polypeptide monomers modified as described). The dimeric form of the IFNγ polypeptides may be provided by the normal association of two monomers or be in the form of a single chain dimeric IFNγ polypeptide. The IFNγ polypeptide described herein may have an in vivo or in vitro bioactivity of the same magnitude as huJFNγ or rhuIFNγ or lower or higher, e.g. an in vivo or in vitro bioactivity of >100% (e.g., 125% or greater, or 150% or greater, or 200% or greater, or 300% or greater, or 400% or greater, or 500% or greater, or 1000% (10-fold) or greater, and so forth), 1-100% of that of huIFNγ or rhuIFNγ, as measured under the same conditions, e.g. 1-25% or 1-50% or 25-100% or 50-100% of that of huIFNγ or rhuIFNγ.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A and 1B show the VH (FIG. 1A, amino acid sequence SEQ ID NO:92, DNA sequence SEQ ID NO:93) and VL (FIG. 1B, amino acid sequence SEQ ID NO:94, DNA sequence SEQ ID NO:95) domains of mAb 225.28s (see also, FIGS. 24 and 25 respectively of WO/2013/050725 A1).

FIG. 2, panels A-H, show the structures of varopis recombinant anti-CSPG4 antibody and IFN fusion proteins. Antibody 9.7.27 V_(H) and V_(L) regions recognizing CSPG4 were engineered in recombinant form with human IgG1 constant regions (panel A), or fused at the C-terminus with native IFNα (panel B), or mutant IFNα that mimics the high receptor affinity and potency of IFNβ (panel C). Panel D illustrates an antibody-IFNβ construct while panel E illustrates an antibody-mutant IFNβ construct. Panels F and G illustrate antibody-IFNgamma and antibody-mutant IFNgamma constructs respectively. It is noted that unlike interferon alpha and interferon beta, IFNγ is typically active as a dimer. In the constructs illustrated in Panels F and G, the interferon attached to each of the heavy chains can dimerize with each other to provide activity. In another illustrative embodiments, shown in panel H, two interferon gammas joined by a linker can be attached to one or to each antibody heavy chain and these can dimerize with each other to provide activity.

FIG. 3 shows expression of CSPG4 on SKMEL-28 and A375 human melanoma cell lines. Cultured cells were harvested by EDTA and stained with anti-CSPG4, rituximab anti-CD20 antibody (ritux), or trastuzumab anti-HER2 antibody (herceptin), followed by anti-kappa-PE staining, and flow cytometry. Controls include anti-kappa-PE or anti-CD20-PE alone.

FIG. 4 shows the sensitivity of human melanoma cell lines to recombinant type 1 interferons (IFNα, IFNβ). Tumor cells were seeded in quadruplicate in a 96 well U-bottom plate at 5×10³ cells/well in RPMI complete medium. Media or graded concentrations of hIFNα or hIFNβ starting at 100 nM or 10 nM respectively and serially diluted 10-fold were added at a final volume of 200 μl/well and incubated at 37° C. in a 5% CO₂ humidified incubator for 72 hours. Cells were pulsed with 1 μCi/well ³[H]-thymidine and harvested 8 hours later. Incorporated radioactivity (counts per minute) was measured using a β-liquid scintillation analyzer and results from 4 replicate cultures reported as arithmetic means±standard deviation.

FIG. 5 shows the sensitivity of human melanoma cell lines to anti-CSPG4-IFNα fusion proteins. Tumor cells were seeded in quadruplicate at 5×10³ cells/well in graded concentrations of unfused parent antibody or fusion protein (starting at 100 nM and serially diluted 10-fold), then incubated for 72 hours. Cells were pulsed with ³[H]-thymidine and harvested 8 hours later. Incorporated radioactivity (counts per minute) was measured and results reported as arithmetic means±SD.

FIG. 6 illustrates inhibition of melanoma cell line proliferation following treatment with IFNα, anti-CSPG4, anti-CSPG4-IFNα or anti-CSPG4-mutIFNα. Quadruplicate samples of cells incubated with the indicated proteins for 48 hours were pulsed for 8 hours with ³[H]-thymidine and its incorporation determined.

FIG. 7 shows the in vivo efficacy of anti-CSPG4, anti-CSPG4-IFNα and anti-CSPG4-mutIFNα against human melanoma xenografts. SCID mice were injected with 2×10⁶ A375 cells s.c. on day 0. Groups of 8 mice were treated with 100 μg protein i.v. on days 5, 12, and 19 and followed for tumor growth and survival. Palpable tumors were bi-directionally measured three times per week using calipers. Animals were sacrificed when their tumors reached 1.4 cm in diameter, per institutional guidelines.

FIG. 8 shows that anti-CSPG4-fusions with IFNγ have differing activities in MTS assays depending on the linker used for the fusion protein on A375 myeloma cells in an MTS assay. Data are expressed as the percent metabolic activity in the treated cells compared with untreated cells. Data are shown as the mean of triplicate samples±SD.

FIG. 9 shows that shows that anti-CSPG4-fusions with IFNγ show differing activities against the T98 glioblastoma in MTS assays depending on the linker used for the fusion protein. Data are expressed as the percent metabolic activity in the treated cells compared with untreated cells. Data are shown as the mean of triplicate samples±SD.

FIG. 10 shows that anti-CSPG4-fusions with IFNγ are more effective than IFNγ in inhibiting the growth the U87 glioblastoma. Data are expressed as the percent metabolic activity in the treated cells compared with untreated cells. Data are shown as the mean of triplicate samples±SD.

FIG. 11 shows that anti-CSPG4-fusions with IFNγ are more effective than IFNγ in inhibiting the growth the U373 glioblastoma. Data are expressed as the percent metabolic activity in the treated cells compared with untreated cells. Data are shown as the mean of triplicate samples±SD.

FIG. 12 shows that anti-CSPG4-fusions with IFNγ are as effective as IFNγ in inhibiting the growth the DBTRG-05 glioblastoma. Data are expressed as the percent metabolic activity in the treated cells compared with untreated cells. Data are shown as the mean of triplicate samples±SD.

FIG. 13 shows that anti-CSPG4 IFNγ fusion proteins upregulate the expression of MHC 1 in glioblastoma cells. U87, U373 and DBTRG-05 glioblastoma cells were incubated with the indicated proteins for 24 hours. They were then detached using 2 mM EDTA, stained with anti-MHC 1, and analyzed by flow cytometry.

FIG. 14 shows that anti-CSPG4-fusions with IFNγ show differing activities against the OVCAR3 ovarian cancer depending on the linker used for the fusion protein. Data are expressed as the percent metabolic activity in the treated cells compared with untreated cells. Data are shown as the mean of triplicate samples±SD.

FIG. 15 shows that anti-CSPG4 IFNγ fusion proteins upregulate the expression of MHC 1 in OVCAR 3 ovarian cells. OVCAR 3 cells were incubated with the indicated proteins for 24 hours. They were then detached using 2 mM EDTA, stained with anti-MHC 1, and analyzed by flow cytometry.

DETAILED DESCRIPTION

Malignant melanoma is an immunogenic, highly aggressive and frequently lethal form of skin cancer. It is the most common cancer in the 17-34 years age group but affects people of all ages, and therefore has a significant socioeconomic impact for patients and their families. Although diagnosed skin lesions can be initially excised by surgical intervention, skin and distal metastases unfortunately occur in 20% of patients originally treated with local disease. Patients with lymph node and other distal metastases have dismal prognosis, and this is partly due to lack of effective treatments for this cohort.

Melanoma has presented major challenges to numerous targeted therapy efforts. While interferons have contemplated for use in the treatment of cancer, the effectiveness of interferons for cancer therapy has been overshadowed by side effects when used at high doses.

In various embodiments, interferons are provided attached to an antibody that binds to chrondroitin sulphate proteoglycan 4 (CSPG4, also known as high molecular weight melanoma associated antigen (HMW-MAA)). In certain embodiments, a type I interferon (e.g., human type I IFN), truncated type I interferon (e.g., truncated human type I IFN), and/or mutant type I interferon (e.g., human mutant type I IFN) fused to the C-terminus of an antibody that binds to CSPG4 is provided. In addition to using wild-type human IFN-alpha (alpha2 and natural higher affinity variants such as alpha 14), interferon beta (IFN-β) and interferon gamma (IFN-γ) as well as mutants and/or truncated forms are also contemplated for use in the anti-CSPG4-IFN constructs contemplated herein. In certain illustrative embodiments, the mutant IFN and alpha 14 are expected to have higher affinity than IFN alpha 2 for the interferon receptor and thus greater anti-tumor efficacy.

As demonstrated in the Examples, recombinant forms of the murine monoclonal antibody 9.2.27 that recognizes CSPG4 (26) were engineered into the backbone of human IgG1 (FIG. 2) and expressed as a fusion protein with interferon. Various constructs included a native form of the antibody, a fusion containing human IFNα, and a fusion containing a mutant IFNα2 (mutIFNα (e.g., IFNα2^(YNS))) that mimics IFNβ in terms of higher affinity binding to IFNAR1 (see, e.g., Eyal et al. (2007) J. Biol. Chem. 282(15): 11602-11611). The recombinant anti-CSPG4 antibody recognized 2 different human melanoma cell lines (SK-MEL-28 and A375) in a specific manner, showing high-level binding (FIG. 3). Both of these cell lines were sensitive to growth inhibition by free, recombinant human IFNα and IFNβ, with IFNβ being more effective, as expected (FIG. 4). The native IgG1 anti-CSPG4 antibody had no effect on the growth of either cell line. The anti-CSPG4-hIFNα fusion was effective at modestly inhibiting the growth of SK-MEL-28 cells, and inhibiting A375 proliferation by 50-60%. By contrast, the anti-CSPG4-mutIFNα fusion potently inhibited the growth of both cell lines, achieving approximately 70% inhibition of SK-MEL-28 and 80% inhibition of A375. Anti-CSPG4-IFNα and anti-CSPG4-mutIFNα also inhibited the proliferation of melanoma cells with different BRAF and NRAS mutations (FIG. 6) with anti-CSPG4-mutIFNα more effective than anti-CSPG4-IFNα.

To test the ability of the fusion proteins to inhibit tumor growth in vivo, A375 cells were grown as subcutaneous xenografts in immunodeficient SCID mice (FIG. 7). After 5 days of tumor growth, mice were treated with a short course (days 5, 12, and 19) of intravenous phosphate buffered saline control, native anti-CSPG4 IgG1, anti-CSPG4-IFNα, or anti-CSPG4-mutIFNα. In mice treated with saline, tumors grew progressively, and all tumors had reached 1.4 cm in diameter before day 40. Treatment with native antibody or anti-CSPG4-IFN appeared to slightly delay tumor growth (all tumors reaching 1.4 cm in diameter by day 44) although these differences were not statistically significant compared to saline control (p=0.073 and p=0.079, respectively). However, treatment with anti-CSPG4mutIFNα significantly delayed the growth of tumors compared to saline control (p=0.003), native antibody (p=0.0048), and anti-CSPG4-IFNα (p=0.0048). Notably, tumors did not appear to progress in mice treated with anti-CSPG4-mutIFNα until after day 19, when therapy was stopped, suggesting ongoing suppression of tumor growth in vivo. Thus, even a brief course of low-dose anti-CSPG4-mutIFNα fusion protein therapy was able to significantly delay the growth a human melanoma in vivo.

Accordingly, it is believed that anti-CSPG4-Interferon constructs are highly potent agents for the inhibition of growth or proliferation of cells that express CSPG4, notably cancer cells that express CSPG4. Such cancers include, but are not limited to melanoma, triple negative breast cancer, and malignant gliomas.

Thus, in certain embodiments, the constructs (e.g., chimeric moieties) comprising an interferon (e.g., IFNα, IFNβ, IFNγ, mutant IFNα, mutant IFNβ, mutant IFNγ, truncated IFNα, truncated IFNβ, truncated IFNγ, etc.) attached to a targeting moiety (e.g., to an antibody that specifically binds CSPG4) are provided. In certain embodiments the constructs include chemical conjugates as well as fusion proteins. Also provided are nucleic acids encoding the fusion proteins (or components thereof) as well as cells transfected with the nucleic acids to express the fusion proteins. Also provided are methods of inhibiting growth and proliferation of cells that express or overexpress CSPG4 using the constructs described herein. In certain embodiments, the cells that express or over express CSPG4 are cancer cells (e.g., melanoma, triple negative breast cancer, malignant glioma, etc.). Accordingly in various embodiments, methods are provided for inhibiting, delaying and/or preventing the growth of a tumor and/or spread of malignant tumor cell using the constructs described herein. In addition, kits comprising the constructs are provided, e.g., for the treatment of various cancers.

I. Constructs Comprising a Targeting Moiety Attached to an Interferon.

It was a surprising discovery that constructs comprising a targeting moiety that binds (e.g., that preferentially or specifically binds) to CSPG4 attached to a native (wildtype) or modified IFN (e.g., mutant IFN-α) can be effectively used to inhibit the growth and/or proliferation of target cells (e.g., cancer cells) that express or overexpress CSPG4. In certain embodiments the CSPG4 targeting moieties are chemically conjugated to the interferon, while in other embodiments, the CSPG4 targeting moiety (or a component thereof) is expressed as a fusion protein with the interferon. When produced as a fusion protein the CSPG4 targeting moiety (e.g., antibody) (or a component thereof) can be directly fused to the interferon or attached by means of a peptide linker (e.g., a (Gly₄Ser)₃ (SEQ ID NO:1) linker, a Gly₄Ser (SEQ ID NO:2) linker, a SerGly₄Ser linker (SEQ ID NO:3), an AEAAAKEAAAKA (SEQ ID NO:4) linker, and the like.

Antibodies that bind to CSPG4

In various embodiments constructs are contemplated comprising an antibody or antibody fragment that binds specifically to CSPG4 attached to an interferon (e.g., full length interferon, biologically active interferon fragment, biologically active interferon mutant, etc.). Antibodies that specifically bind CSPG4 are known to those of skill in the art and a number of different monoclonal antibodies that specifically bind CSPG4 have been produced (see, e.g., PCT Publication WO/2010/033866, and the like).

In addition, anti-CSPG4 antibodies can be made using methods well known to those of skill in the art. For example, antibodies can be produced by immunizing an animal with CSPG4 or an immunogenic fragment thereof and raising the antibodies in that animal. Polyclonal antibodies can be recovered and used or converted to monoclonal antibodies according to methods well known to those of skill in the art.

CSPG4 is a well known and well characterized protein. In one embodiment, has an amino acid sequence set forth as:

(SEQ ID NO: 5)       10      20      30      40      50      60 MQSGPRPPLP APGLALALTL TMLARLASAA SFFGENHLEV PVATALTDID LQLQFSTSQP       70      80      90      100     110       120 EALLLLAAGP ADHLLLQLYS GRLQVRLVLG QEELRLQTPA ETLLSDSIPH TVVLTVVEGW      130      140      150      160      170      180 ATLSVDGFLN ASSAVPGAPL EVPYGLFVGG TGTLGLPYLR GTSRPLRGCL HAATLNGRSL      190      200      210      220      230      240 LRPLTPDVHE GCAEEFSASD DVALGFSGPH SLAAFPAWGT QDEGTLEFTL TTQSRQAPLA      250      260      270      280      290      300 FQAGGRRGDF IYVDIFEGHL RAVVEKGQGT VLLHNSVPVA DGQPHEVSVH INAHRLEISV      310      320      330      340      350      360 DQYPTHTSNR GVLSYLEPRG SLLLGGLDAE ASRHLQEHRL GLTPEATNAS LLGCMEDLSV      370      380      390      400      410      420 NGQRRGLREA LLTRNMAAGC RLEEEEYEDD AYGHYEAFST LAPEAWPAME LPEPCVPEPG      430      440      450      460      470      480 LPPVFANFTQ LLTISPLVVA EGGTAWLEWR HVQPTLDLME AELRKSQVLF SVTRGARHGE      490      500      510      520      530      540 LELDIPGAQA RKMFTLLDVV NRKARFIHDG SEDTSDQLVL EVSVTARVPM PSCLRRGQTY      550      560      570      580      590      600 LLPIQVNPVN DPPHIIFPHG SLMVILEHTQ KPLGPEVFQA YDPDSACEGL TFQVLGTSSG      610      620      630      640      650      660 LPVERRDQPG EPATEFSCRE LEAGSLVYVH RGGPAQDLTF RVSDGLQASP PATLKVVAIR      670      680      690      700      710      720 PAIQIHRSTG LRLAQGSAMP ILPANLSVET NAVGQDVSVL FRVTGALQFG ELQKQGAGGV      730      740      750      760      770      780 EGAEWWATQA FHQRDVEQGR VRYLSTDPQH HAYDTVENLA LEVQVGQEIL SNLSFPVTIQ      790      800      810      820      830      840 RATVWMLRLE PLHTQNTQQE TLTTAHLEAT LEEAGPSPPT FHYEVVQAPR KGNLQLQGTR      850      860      870      880      890      900 LSDGQGFTQD DIQAGRVTYG ATARASEAVE DTFRFRVTAP PYFSPLYTFP IHIGGDPDAP      910      920      930      940      950      960 VLTNVLLVVP EGGEGVLSAD HLFVKSLNSA SYLYEVMERP RHGRLAWRGT QDKTTMVTSF      970      980      990     1000     1010     1020 TNEDLLRGRL VYQHDDSETT EDDIPFVATR QGESSGDMAW EEVRGVFRVA IQPVNDHAPV     1030     1040     1050     1060     1070     1080 QTISRIFHVA RGGRRLLTTD DVAFSDADSG FADAQLVLTR KDLLFGSIVA VDEPTRPIYR     1090     1100     1110     1120     1130     1140 FTQEDLRKRR VLFVHSGADR GWIQLQVSDG QHQATALLEV QASEPYLRVA NGSSLVVPQG     1150     1160     1170     1180     1190     1200 GQGTIDTAVL HLDTNLDIRS GDEVHYHVTA GPRWGQLVRA GQPATAFSQQ DLLDGAVLYS     1210     1220     1230     1240     1250     1260 HNGSLSPRDT MAFSVEAGPV HTDATLQVTI ALEGPLAPLK LVRHKKIYVF QGEAAEIRRD     1270     1280     1290     1300     1310     1320 QLEAAQEAVP PADIVFSVKS PPSAGYLVMV SRGALADEPP SLDPVQSFSQ EAVDTGRVLY     1330     1340     1350     1360     1370     1380 LHSRPEAWSD AFSLDVASGL GAPLEGVLVE LEVLPAAIPL EAQNFSVPEG GSLTLAPPLL     1390     1400     1410     1420     1430     1440 RVSGPYFPTL LGLSLQVLEP PQHGALQKED GPQARTLSAF SWRMVEEQLI RYVHDGSETL     1450     1460     1470     1480     1490     1500 TDSFVLMANA SEMDRQSHPV AFTVTVLPVN DQPPILTTNT GLQMWEGATA PIPAEALRST     1510     1520     1530     1540     1550     1560 DGDSGSEDLV YTIEQPSNGR VVLRGAPGTE VRSFTQAQLD GGLVLFSHRG TLDGGFRFRL     1570     1580     1590     1600     1610     1620 SDGEHTSPGH FFRVTAQKQV LLSLKGSQTL TVCPGSVQPL SSQTLRASSS AGTDPQLLLY     1630     1640     1650     1660     1670     1680 RVVRGPQLGR LFHAQQDSTG EALVNFTQAE VYAGNILYEH EMPPEPFWEA HDTLELQLSS     1690     1700     1710     1720     1730     1740 PPARDVAATL AVAVSFEAAC PQRPSHLWKN KGLWVPEGQR ARITVAALDA SNLLASVPSP     1750     1760     1770     1780     1790     1800 QRSEHDVLFQ VTQFPSRGQL LVSEEPLHAG QPHFLQSQLA AGQLVYAHGG GGTQQDGFHF     1810     1820     1830     1840     1850     1860 RAHLQGPAGA SVAGPQTSEA FAITVRDVNE RPPQPQASVP LRLTRGSRAP ISRAQLSVVD     1870     1880     1890     1900     1910     1920 PDSAPGEIEY EVQRAPHNGF LSLVGGGLGP VTRFTQADVD SGRLAFVANG SSVAGIFQLS     1930     1940     1950     1960     1970     1980 MSDGASPPLP MSLAVDILPS AIEVQLRAPL EVPQALGRSS LSQQQLRVVS DREEPEAAYR     1990     2000     2010     2020     2030     2040 LIQGPQYGHL LVGGRPTSAF SQFQIDQGEV VFAFTNFSSS HDHFRVLALA RGVNASAVVN     2050     2060     2070     2080     2090     2100 VTVRALLHVW AGGPWPQGAT LRLDPTVLDA GELANRTGSV PRFRLLEGPR HGRVVRVPRA     2110     2120     2130     2140     2150     2160 RTEPGGSQLV EQFTQQDLED GRLGLEVGRP EGRAPGPAGD SLTLELWAQG VPPAVASLDF     2170     2180     2190     2200     2210     2220 ATEPYNAARP YSVALLSVPE AARTEAGKPE SSTPTGEPGP MASSPEPAVA KGGFLSFLEA     2230     2240     2250     2260     2270     2280 NMFSVIIPMC LVLLLLALIL PLLFYLRKRN KTGKHDVQVL TAKPRNGLAG DTETFRKVEP     2290     2300     2310     2320 GQAIPLTAVP GQGPPPGGQP DPELLQFCRT PNPALKNGQY WV  See, also, GENBANK Accession No. Q6UVK1 incorporated herein by reference (note leader is underlined in above sequence).

In certain embodiments the CSPG4 protein can be encoded by the nucleic acid sequence set forth as:

(SEQ ID NO: 6)    1 gcgcccagga gcagagccgc gctcgctcca ctcagctccc agctcccagg actccgctgg   61 ctcctcgcaa gtcctgccgc ccagcccgcc gggatgcagt ccgggccgcg gcccccactt  121 ccagcccccg gcctggcctt ggctttgacc ctgactatgt tggccagact tgcatccgcg  181 gcttccttct tcggtgagaa ccacctggag gtgcctgtgg ccacggctct gaccgacata  241 gacctgcagc tgcagttctc cacgtcccag cccgaagccc tccttctcct ggcagcaggc  301 ccagctgacc acctcctgct gcagctctac tctggacgcc tgcaggtcag acttgttctg  361 ggccaggagg agctgaggct gcagactcca gcagagacgc tgctgagtga ctccatcccc  421 cacactgtgg tgctgactgt cgtagagggc tgggccacgt tgtcagtcga tgggtttctg  481 aacgcctcct cagcagtccc aggagccccc ctagaggtcc cctatgggct ctttgttggg  541 ggcactggga cccttggcct gccctacctg aggggaacca gccgacccct gaggggttgc  601 ctccatgcag ccaccctcaa tggccgcagc ctcctccggc ctctgacccc cgatgtgcat  661 gagggctgtg ctgaagagtt ttctgccagt gatgatgtgg ccctgggctt ctctgggccc  721 cactctctgg ctgccttccc tgcctggggc actcaggacg aaggaaccct agagtttaca  781 ctcaccacac agagccggca ggcacccttg gccttccagg cagggggccg gcgtggggac  841 ttcatctatg tggacatatt tgagggccac ctgcgggccg tggtggagaa gggccagggt  901 accgtattgc tccacaacag tgtgcctgtg gccgatgggc agccccatga ggtcagtgtc  961 cacatcaatg ctcaccggct ggaaatctcc gtggaccagt accctacgca tacttcgaac 1021 cgaggagtcc tcagctacct ggagccacgg ggcagtctcc ttctcggggg gctggatgca 1081 gaggcctctc gtcacctcca ggaacaccgc ctgggcctga caccagaggc caccaatgcc 1141 tccctgctgg gctgcatgga agacctcagt gtcaatggcc agaggcgggg gctgcgggaa 1201 gctttgctga cgcgcaacat ggcagccggc tgcaggctgg aggaggagga gtatgaggac 1261 gatgcctatg gacattatga agctttctcc accctggccc ctgaggcttg gccagccatg 1321 gagctgcctg agccatgcgt gcctgagcca gggctgcctc ctgtctttgc caatttcacc 1381 cagctgctga ctatcagccc actggtggtg gccgaggggg gcacagcctg gcttgagtgg 1441 aggcatgtgc agcccacgct ggacctgatg gaggctgagc tgcgcaaatc ccaggtgctg 1501 ttcagcgtga cccgaggggc acgccatggc gagctcgagc tggacatccc gggagcccag 1561 gcacgaaaaa tgttcaccct cctggacgtg gtgaaccgca aggcccgctt catccacgat 1621 ggctctgagg acacctccga ccagctggtg ctggaggtgt cggtgacggc tcgggtgccc 1681 atgccctcat gccttcggag gggccaaaca tacctcctgc ccatccaggt caaccctgtc 1741 aatgacccac cccacatcat cttcccacat ggcagcctca tggtgatcct ggaacacacg 1801 cagaagccgc tggggcctga ggttttccag gcctatgacc cggactctgc ctgtgagggc 1861 ctcaccttcc aggtccttgg cacctcctct ggcctccccg tggagcgccg agaccagcct 1921 ggggagccgg cgaccgagtt ctcctgccgg gagttggagg ccggcagcct agtctatgtc 1981 caccgcggtg gtcctgcaca ggacttgacg ttccgggtca gcgatggact gcaggccagc 2041 cccccggcca cgctgaaggt ggtggccatc cggccggcca tacagatcca ccgcagcaca 2101 gggttgcgac tggcccaagg ctctgccatg cccatcttgc ccgccaacct gtcggtggag 2161 accaatgccg tggggcagga tgtgagcgtg ctgttccgcg tcactggggc cctgcagttt 2221 ggggagctgc agaagcaggg ggcaggtggg gtggagggtg ctgagtggtg ggccacacag 2281 gcgttccacc agcgggatgt ggagcagggc cgcgtgaggt acctgagcac tgacccacag 2341 caccacgctt acgacaccgt ggagaacctg gccctggagg tgcaggtggg ccaggagatc 2401 ctgagcaatc tgtccttccc agtgaccatc cagagagcca ctgtgtggat gctgcggctg 2461 gagccactgc acactcagaa cacccagcag gagaccctca ccacagccca cctggaggcc 2521 accctggagg aggcaggccc aagcccccca accttccatt atgaggtggt tcaggctccc 2581 aggaaaggca accttcaact acagggcaca aggctgtcag atggccaggg cttcacccag 2641 gatgacatac aggctggccg ggtgacctat ggggccacag cacgtgcctc agaggcagtc 2701 gaggacacct tccgtttccg tgtcacagct ccaccatatt tctccccact ctataccttc 2761 cccatccaca ttggtggtga cccagatgcg cctgtcctca ccaatgtcct cctcgtggtg 2821 cctgagggtg gtgagggtgt cctctctgct gaccacctct ttgtcaagag tctcaacagt 2881 gccagctacc tctatgaggt catggagcgg ccccgccatg ggaggttggc ttggcgtggg 2941 acacaggaca agaccactat ggtgacatcc ttcaccaatg aagacctgtt gcgtggccgg 3001 ctggtctacc agcatgatga ctccgagacc acagaagatg atatcccatt tgttgctacc 3061 cgccagggcg agagcagtgg tgacatggcc tgggaggagg tacggggtgt cttccgagtg 3121 gccatccagc ccgtgaatga ccacgcccct gtgcagacca tcagccggat cttccatgtg 3181 gcccggggtg ggcggcggct gctgactaca gacgacgtgg ccttcagcga tgctgactcg 3241 ggctttgctg acgcccagct ggtgcttacc cgcaaggacc tcctctttgg cagtatcgtg 3301 gccgtagatg agcccacgcg gcccatctac cgcttcaccc aggaggacct caggaagagg 3361 cgagtactgt tcgtgcactc aggggctgac cgtggctgga tccagctgca ggtgtccgac 3421 gggcaacacc aggccactgc gctgctggag gtgcaggcct cggaacccta cctccgtgtg 3481 gccaacggct ccagccttgt ggtccctcaa ggaggccagg gcaccatcga cacggccgtg 3541 ctccacctgg acaccaacct cgacatccgc agtggggatg aggtccacta ccacgtcaca 3601 gctggccctc gctggggaca gctagtccgg gctggtcagc cagccacagc cttctcccag 3661 caggacctgc tggatggggc cgttctctat agccacaatg gcagcctcag cccccgcgac 3721 accatggcct tctccgtgga agcagggcca gtgcacacgg atgccaccct acaagtgacc 3781 attgccctag agggcccact ggccccactg aagctggtcc ggcacaagaa gatctacgtc 3841 ttccagggag aggcagctga gatcagaagg gaccagctgg aggcagccca ggaggcagtg 3901 ccacctgcag acatcgtatt ctcagtgaag agcccaccga gtgccggcta cctggtgatg 3961 gtgtcgcgtg gcgccttggc agatgagcca cccagcctgg accctgtgca gagcttctcc 4021 caggaggcag tggacacagg cagggtcctg tacctgcact cccgccctga ggcctggagc 4081 gatgccttct cgctggatgt ggcctcaggc ctgggtgctc ccctcgaggg cgtccttgtg 4141 gagctggagg tgctgcccgc tgccatccca ctagaggcgc aaaacttcag cgtccctgag 4201 ggtggcagcc tcaccctggc ccctccactg ctccgtgtct ccgggcccta cttccccact 4261 ctcctgggcc tcagcctgca ggtgctggag ccaccccagc atggagccct gcagaaggag 4321 gacggacctc aagccaggac cctcagcgcc ttctcctgga gaatggtgga agagcagctg 4381 atccgctacg tgcatgacgg gagcgagaca ctgacagaca gttttgtcct gatggctaat 4441 gcctccgaga tggatcgcca gagccatcct gtggccttca ctgtcactgt cctgcctgtc 4501 aatgaccaac cccccatcct cactacaaac acaggcctgc agatgtggga gggggccact 4561 gcgcccatcc ctgcggaggc tctgaggagc acggacggcg actctgggtc tgaggatctg 4621 gtctacacca tcgagcagcc cagcaacggg cgggtagtgc tgcggggggc gccgggcact 4681 gaggtgcgca gcttcacgca ggcccagctg gacggcgggc tcgtgctgtt ctcacacaga 4741 ggaaccctgg atggaggctt ccgcttccgc ctctctgacg gcgagcacac ttcccccgga 4801 cacttcttcc gagtgacggc ccagaagcaa gtgctcctct cgctgaaggg cagccagaca 4861 ctgactgtct gcccagggtc cgtccagcca ctcagcagtc agaccctcag ggccagctcc 4921 agcgcaggca ctgaccccca gctcctgctc taccgtgtgg tgcggggccc ccagctaggc 4981 cggctgttcc acgcccagca ggacagcaca ggggaggccc tggtgaactt cactcaggca 5041 gaggtctacg ctgggaatat tctgtatgag catgagatgc cccccgagcc cttttgggag 5101 gcccatgata ccctagagct ccagctgtcc tcgccgcctg cccgggacgt ggccgccacc 5161 cttgctgtgg ctgtgtcttt tgaggctgcc tgtccccagc gccccagcca cctctggaag 5221 aacaaaggtc tctgggtccc cgagggccag cgggccagga tcaccgtggc tgctctggat 5281 gcctccaatc tcttggccag cgttccatca ccccagcgct cagagcatga tgtgctcttc 5341 caggtcacac agttccccag ccggggccag ctgttggtgt ccgaggagcc cctccatgct 5401 gggcagcccc acttcctgca gtcccagctg gctgcagggc agctagtgta tgcccacggc 5461 ggtgggggca cccagcagga tggcttccac tttcgtgccc acctccaggg gccagcaggg 5521 gcctccgtgg ctggacccca aacctcagag gcctttgcca tcacggtgag ggatgtaaat 5581 gagcggcccc ctcagccaca ggcctctgtc ccactccggc tcacccgagg ctctcgtgcc 5641 cccatctccc gggcccagct gagtgtggtg gacccagact cagctcctgg ggagattgag 5701 tacgaggtcc agcgggcacc ccacaacggc ttcctcagcc tggtgggtgg tggcctgggg 5761 cccgtgaccc gcttcacgca agccgatgtg gattcagggc ggctggcctt cgtggccaac 5821 gggagcagcg tggcaggcat cttccagctg agcatgtctg atggggccag cccacccctg 5881 cccatgtccc tggctgtgga catcctacca tccgccatcg aggtgcagct gcgggcaccc 5941 ctggaggtgc cccaagcttt ggggcgctcc tcactgagcc agcagcagct ccgggtggtt 6001 tcagatcggg aggagccaga ggcagcatac cgcctcatcc agggacccca gtatgggcat 6061 ctcctggtgg gcgggcggcc cacctcggcc ttcagccaat tccagataga ccagggcgag 6121 gtggtctttg ccttcaccaa cttctcctcc tctcatgacc acttcagagt cctggcactg 6181 gctaggggtg tcaatgcatc agccgtagtg aacgtcactg tgagggctct gctgcatgtg 6241 tgggcaggtg ggccatggcc ccagggtgcc accctgcgcc tggaccccac cgtcctagat 6301 gctggcgagc tggccaaccg cacaggcagt gtgccgcgct tccgcctcct ggagggaccc 6361 cggcatggcc gcgtggtccg cgtgccccga gccaggacgg agcccggggg cagccagctg 6421 gtggagcagt tcactcagca ggaccttgag gacgggaggc tggggctgga ggtgggcagg 6481 ccagagggga gggcccccgg ccccgcaggt gacagtctca ctctggagct gtgggcacag 6541 ggcgtcccgc ctgctgtggc ctccctggac tttgccactg agccttacaa tgctgcccgg 6601 ccctacagcg tggccctgct cagtgtcccc gaggccgccc ggacggaagc agggaagcca 6661 gagagcagca cccccacagg cgagccaggc cccatggcat ccagccctga gcccgctgtg 6721 gccaagggag gcttcctgag cttccttgag gccaacatgt tcagcgtcat catccccatg 6781 tgcctggtac ttctgctcct ggcgctcatc ctgcccctgc tcttctacct ccgaaaacgc 6841 aacaagacgg gcaagcatga cgtccaggtc ctgactgcca agccccgcaa cggcctggct 6901 ggtgacaccg agacctttcg caaggtggag ccaggccagg ccatcccgct cacagctgtg 6961 cctggccagg ggccccctcc aggaggccag cctgacccag agctgctgca gttctgccgg 7021 acacccaacc ctgcccttaa gaatggccag tactgggtgt gaggcctggc ctgggcccag 7081 atgctgatcg ggccagggac aggcttgccc atgtcccggg ccccattgct tccatgcctg 7141 gtgctgtctg agtatcccca gagcaagaga gacctggaga caccaggggt ggagggtcct 7201 gggagatagt cccaggggtc cgggacagag tggagtcaag agctggaacc tccctcagct 7261 cactccgagc ctggagaact gcaggggcca aggtggaggc aggcttaagt tcagtcctcc 7321 tgccctggag ctggtttggg ctgtcaaaac cagggtaacc tcctacatgg gtcatgactc 7381 tgggtcctgg gtctgtgacc ttgggtaagt cgcgcctgac ccaggctgct aagagggcaa 7441 ggagaaggaa gtaccctggg gagggaaggg acagaggaag ctattcctgg cttttccact 7501 ccaacccagg ccaccctttg tctctgcccc agagttgaga aaaaaacttc ctcccctggt 7561 tttttaggga gatggtatcc cctggagtag agggcaagag gagagagcgc ctccagtcta 7621 gaaggcataa gccaatagga taatatattc agggtgcagg gtgggtaggt tgctctgggg 7681 atgggtttat ttaagggaga ttgcaaggaa gctatttaac atggtgctga gctagccagg 7741 actgatggag cccctggggg tgtgggatgg aggagggtct gcagccagtt cattcccagg 7801 gccccatctt gatgggccaa gggctaaaca tgcatgtgtc agtggctttg gagcaggtta 7861 ggctggggct catcgagggt ctcaggccga ggccactgcg gtgccagtgc ccccctgagg 7921 actagggcag gcagctgggg gcacttggtt ccatggagcc tggataaaca gtgctttgga 7981 ggctctggac agctgtgtgg tgtttgtgtc ttaactatgc actgggccct tgtctgcgtc 8041 ggcttgcata cagagggccc ctggggtcgg ccctccggcc tggcctcagc cagtgggatg 8101 gacagggcca ggcaggcctc tgaacttcca cctcctgggg cctcccagac ctcctgtgcc 8161 cccacctgtg tgggcaggtg ggccagtctt cgggtgatgg gaccaaaccc cttcagttca 8221 gtagagaaag gctaggtcct ctacaaagag ctgcaagaca aaaattaaaa taaatgctcc 8281 ccaccctaga aaaaaaaaaa aaaaa See also Genebank ACCESSION No: NM_001897, which is incorporated herein by reference.

One of skill in the art can readily use this or other nucleic acid sequences encoding CSPG4 to produce a CSPG4 polypeptide (e.g., for use in raising antibodies) using standard methods in molecular biology (see, e.g., Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).

In certain embodiments, single chain anti-CSPG4 antibodies can be created using a phage display library. One such method is described by Fernandez et al. (2005) J. Clin. Oncol., ASCO Annual Meeting Proceedings. 23(16S), Part I of II (June 1 Supplement), 2005: 2550. The authors used combinatorial immunoglobulin (Ig) libraries with phage display to generate in vitro human Ig Fab fragments without the need to maintain on-going hybridoma culture. A library of 10¹⁰ clones from the cDNA of peripheral blood mononuclear cells of patients with adenocarcinoma was used to identify target-specific Ig. Generally following removal of non-specific Fabs by exposing the Ig library to the epithelial cell line HEK, target-specific antibodies were selected by exposing the Fab library to HEK transduced with the target protein. Six rounds of selection resulted in a panel of target specific phage. Similar methods can readily be used to produce anti-CSPG4 antibodies in addition to the antibodies described below.

In various embodiments, the anti-CSPG4 antibodies used in the constructs described herein specifically bind to CSPG4 to form an immune complex. Typically the antibody comprises an antigen-binding region (e.g. one or more variable regions, or one to 6 CDRs) derived from an antibody that is known to bind CSPG4, preferably human CSPG4.

In certain embodiments the antibody binds to CSPG4 with an affinity greater than (K_(D) less than) about 1×10⁻⁶ M, or a K_(D) less than about 1×10⁻⁷ M, or a K_(D) less than about 1×10⁻⁸ M or a K_(D) less than about 1×10⁻⁹ M, or a K_(D) less than about 1×10⁻¹⁰ M, or a K_(D) less than about 1×10⁻¹¹ M, or a K_(D) less than about 1×10⁻¹² M as measured using a BiaCore®.

Antibodies that bind to CSPG4 are disclosed, for example, in WO 1989/011296. Such antibodies include mouse monoclonal antibodies 225.28, 225.28s; 763.74; VF1-TP41.2; VT80.1 12; 653.25; 763.74; TP61.5, and T8-203 (see e.g., WO 1989/11296; Drake et al. (2009) Cancer Immunol. Immunother., 58(3): 415-427; Goto et al. (2008) Clin. Cancer Res. 14: 3401-3407), 9.2.27 (see, e.g., Morgan et al. (1981) Hybridoma, 1: 27-36) single chain antibodies 149.53, 225.28, 763.74, TP61.5, VF1-TP34, and VF1-TP41.2 (see, e.g., Campoli et al. (2004) Crit. Rev. Immunol., 24: 267-296 and Wang et al. (2011) Cancer Res., 71(24): 7410-7422), MEL-14, MEL-5 (see, e.g., U.S. Patent Publication No: 2010/0047164), and the like.

In certain embodiments the antibodies used in the CSPG4-interferon constructs contemplated herein comprise at least one, or at least two, or three complementarity determining regions (CDRs) from the VH region of an antibody determined to specifically bind CSPG4 (e.g., an antibody shown in Table 1) and/or at least one, or at least two, or three complementarity determining regions (CDRs) from the VL region of an antibody determined to specifically bind CSPG4 (e.g., an antibody shown in Table 1). In certain embodiments the antibody comprises a variable region (e.g. a heavy chain variable domain (VH) and/or a light chain variable domain (VL)) region of an antibody determined to specifically bind CSPG4 (e.g., an antibody shown in Table 1).

The amino acid sequences of these antibodies are well known to those of skill in the art. For example, European Patent Publication No: EP 0411893 A2 gives the amino acid sequence of the 9.2.27 VL domain as:

(SEQ ID NO: 7) NIVLTQSPAS LAVSLGQRAT ISCRASESVD SYGNSFMHWY QQKPGQPPKL LIYLASNLES GVPARFSGSG SRTDFTLTID PVEADDAATY YCQQNNEDPL TFGSGTKLEI KR and the 9.2.27 VH domain as:

(SEQ ID NO: 8) QVQLQQSGPE LVKPGASVKI SCKASGYAFS RSWMNWVKQR PGQGLEWIGR IYPGDGDTNY NGKFKGKATL TADKSSSTAY MQVSSLTSVD SAVYFCARGN TVVVPYTMDY WGQGTSVTVS S Similarly, the amino acid sequences of the VH and VL domains of mAb 225.28s are shown in FIGS. 24 and 25 respectively of WO/2013/050725 A1, reproduced herein as FIGS. 1A and 1B, respectively.

In certain embodiments the antibody is a chimeric, humanized, or fully human antibody (or fragment thereof) that binds the epitope bound by any one or more of the antibodies shown in Table 1. In certain embodiments the antibody is a chimeric, humanized, or fully human antibody (or fragment thereof) derived from any one or more of the antibodies shown in Table 1 (e.g., derived from 9.2.27).

In certain embodiments the antibody comprises one or more human constant regions, e.g. one or more human heavy chain constant domains (e.g. E constant domains) and/or a human light chain (e.g. K or X) constant domain. In certain embodiments the antibody comprises one or more human framework regions within the VH and/or VL domains.

In certain embodiments the sequence of the humanized immunoglobulin heavy chain variable region framework and/or the humanized light chain variable region framework can be at least about 65%, or at least about 75%, or at least about 85% m or at least about 90%, or at least about 95%, or at least about 98%, or at least about 99% identical to the sequence of the donor immunoglobulin heavy chain and/or light chain variable region framework respectively. Human framework regions, and mutations that can be made in a humanized antibody framework regions, are known in the art (see, for example, U.S. Pat. No. 5,585,089).

In another embodiment, the antibody comprises one or more variable regions capable of binding specifically to CSPG4, and one or more constant regions. In certain embodiments the antibody is a chimeric antibody, a humanized antibody or a human antibody. In one embodiment, the antibody comprises one or more variable domains derived from an igE isotype. In certain embodiments the antibody comprises one or more variable domains derived from an immunoglobulin isotype other than IgE (e.g., IgA, IgD, IgG or IgM, for example IgG1), and one or more constant domains derived from an immunoglobulin of the isotype IgE or another isotype.

In certain embodiments the antibody comprises one or more complementarity-determining regions (CDRs) derived from an immunoglobulin isotype other than IgE (e.g., IgA, IgD, IgG or IgM, for example IgG1), and one or more framework regions and or constant domains derived from an immunoglobulin of the isotype IgE. For instance, in certain embodiments, the antibody may comprise one or more variable domains or complementarity-determining regions (CDRs) derived from an IgG, e.g. IgG1.

In certain embodiments the variable domains or CDRs are derived from a first mammalian species, and the framework regions and/or constant domains are derived from a second mammalian species different to the first mammalian species. In one embodiment, the variable regions or CDRs are derived from a non-human species, e.g. a mouse and the framework regions and/or constant domains are human.

TABLE 1 Illustrative combinations of antibodies and interferons for the anti-CSPG4-Interferon constructs described herein. It will be recognized that the antiboides can comprise full-length listed antibodies or CDRs derived from the VH and/or the VL domain of these antibodies. The interferons can comprise the native interferons, truncated interferons, mutant interferons, and the like. mutIFN indicates a mutant interferon, e.g., such as the mutant interferons described below. Antibody Attached Interferon 9.2.27 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 225.28 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 225.28s IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 763.74 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ VF1- IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ TP41.2 VT80.1 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 653.25 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 763.74 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ TP61.5 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ T8-203 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 149.53 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 225.28 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ 763.74 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ TP61.5 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ VF1-TP34 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ VF1- IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ TP41.2 MEL-14 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ MEL-5 IFN-α IFN-β IFN-γ mutIFN-α mutIFN-β mutIFN-γ

Antibodies against CSPG4 sequences can be generated by well-established methods, and at least the variable regions or CDRs from such antibodies may be used in the constructs described herein. Thus, for example, the generated antibodies may be used to donate CDR or variable region sequences into IgE (or other isotype) acceptor sequences. Methods for synthesizing polypeptides and immunizing a host animal are well known in the art. Typically, the host animal (e.g. a mouse) is inoculated intraperitoneally with an amount of immunogen (e.g., CSPG4 or a polypeptide comprising an immunogenic fragment thereof), and (in the case of monoclonal antibody production) hybridomas prepared from its lymphocytes and immortalized myeloma cells using, for example, the general somatic cell hybridization technique of Kohler and Milstein (1975) Nature, 25(6):495-497.

Hybridomas that produce suitable antibodies can be grown in vitro or in vivo using known procedures. Monoclonal antibodies can be isolated from the culture media or body fluids, by conventional immunoglobulin purification procedures such as ammonium sulfate precipitation, gel electrophoresis, dialysis, chromatography, and ultrafiltration, if desired. Undesired activity if present can be removed, for example, by running the preparation over adsorbents made of the immunogen attached to a solid phase and eluting or releasing the desired antibodies off the immunogen. If desired, the antibody (monoclonal or polyclonal) of interest may be sequenced and the polynucleotide sequence may then be cloned into a vector for expression or propagation. The sequence encoding the antibody may be maintained in a vector in a host cell and the host cell can then be expanded and frozen for future use.

Phage display technology, for instance as described in U.S. Pat. No. 5,565,332 and other published documents, may be used to select and produce human antibodies and antibody fragments in vitro, from immunoglobulin variable (V) domain gene repertoires from unimmunized donors (e.g., from human subjects, including patients suffering from a relevant disorder). For example, existing antibody phage display libraries may be panned in parallel against a large collection of synthetic polypeptides. According to this technique, antibody V domain genes are cloned in-frame into either a major or minor coat protein gene of a filamentous bacteriophage, such as F1, M13 or fd, and displayed as functional antibody fragments on the surface of the phage particle. Because the filamentous particle contains a single-stranded DNA copy of the phage genome, selections based on the functional properties of the antibody also result in selection of the gene encoding the antibody exhibiting those properties. Thus antibody sequences selected using phage display from human libraries may include human CDR or variable region sequences conferring specific binding to CSPG4, which may be used to provide fully human antibodies for use in constructs described herein.

Methods for deriving heavy and light chain sequences from human B cell and plasma cell clones are also well known in the art and typically performed using polymerase chain reaction (PGR) techniques, examples of the methods are described in: Kuppers (2004) Meth. Mol. Biol., 271: 225-238; Yoshioka et al. (2011) BMC Biotechnol. 11: 75; Scheeren et al. (2011) PLoS ONE, 6(4): e17189. Wrammert et al. (2008) Nature, 453: 667-671; Kurosawa et al. (2011) BMC Biotechnol. 11: 39; Tiller et al. (2007) J. Immunol. Meth., 329(1-2): 112-124. Thus, in various embodiments antibody sequences selected using B cell clones may include human CDR or variable region sequences conferring specific binding to CSPG4, which may be used to provide fully human antibodies for use in the present invention.

Using the known sequences for the various anti-CSPG4 antibodies (e.g., the antibodies listed in Table 1, such as 9.2.27), a variety of phage display (or yeast display) methods can be used to generate other antibodies that specifically bind CSPG4 with the same or even greater affinity.

Chain Shuffling Methods.

One approach to creating antibody variants has been to replace the original VH or VL gene with a repertoire of V-genes to create new partners (chain shuffling) (Clackson et al. (1991) Nature. 352: 624-628) in a phage display or yeast display library. Using chain shuffling and phage display, the affinity of a human scFv antibody fragment that bound the hapten phenyloxazolone (phOx) was increased from 300 nM to 1 nM (300 fold) (Marks et al. (1992) Bio/Technology 10: 779-783).

Thus, for example, to alter the affinity of an anti-CSPG4 antibody (e.g., the 9.2.27 antibody), a mutant scFv gene repertoire can be created containing a V_(H) gene of the prototypic 9.2.27 antibody and a human V_(L) gene repertoire (light chain shuffling). The scFv gene repertoire can be cloned into a phage display vector, e.g., pHEN-1 (Hoogenboom et al. (1991) Nucleic Acids Res., 19: 4133-4137) or other vectors, and after transformation a library of transformants is obtained.

Similarly, for heavy chain shuffling, a mutant scFv gene repertoire can be created containing a V_(L) gene of the prototypic 9.2.27 antibody and a human V_(H) gene repertoire (heavy chain shuffling). The scFv gene repertoire can be cloned into a phage display vector, e.g., pHEN-1 (Hoogenboom et al. (1991) Nucleic Acids Res., 19: 4133-4137) or other vectors, and after transformation a library of transformants is obtained.

The resulting libraries can be screened against the relevant target (e.g., CSPG4) and/or for cross-reactivity with one or more of the antibodies shown in Table 1.

Site-Directed Mutagenesis to Improve Binding Affinity.

The majority of antigen contacting amino acid side chains are typically located in the complementarity determining regions (CDRs), three in the V_(H) (CDR1, CDR2, and CDR3) and three in the V_(L) (CDR1, CDR2, and CDR3) (Chothia et al. (1987) J. Mol. Biol., 196: 901-917; Chothia et al. (1986) Science, 233: 755-8; Nhan et al. (1991) J. Mol. Biol., 217: 133-151). These residues contribute the majority of binding energetics responsible for antibody affinity for antigen. In other molecules, mutating amino acids that contact the target ligand has been shown to be an effective means of increasing the affinity of one protein molecule for its binding partner (Lowman et al. (1993) J. Mol. Biol., 234: 564-578; Wells (1990) Biochemistry, 29: 8509-8516). Site-directed mutagenesis of CDRs and screening against the target, in particular for binding at CSPG4, can produce antibodies having improved binding affinity.

CDR Randomization to Produce Higher Affinity Human scFv.

In an extension of simple site-directed mutagenesis, mutant antibody libraries can be created where partial or entire CDRs are randomized (V_(L) CDR1 CDR2 and/or CDR3 and/or V_(H) CDR1, CDR2 and/or CDR3). In one embodiment, each CDR is randomized in a separate library, using a known antibody (e.g., 9.2.27 or one or more of the other antibodies in Table 1) as a template. The CDR sequences of the highest affinity mutants from each CDR library are combined to obtain an additive increase in affinity. A similar approach has been used to increase the affinity of human growth hormone (hGH) for the growth hormone receptor over 1500 fold from 3.4×10⁻¹⁰ to 9.0×10⁻¹³ M (Lowman et al. (1993) J. Mol. Biol., 234: 564-578).

V_(H) CDR3 often occupies the center of the binding pocket, and thus mutations in this region are likely to result in an increase in affinity (Clackson et al. (1995) Science, 267: 383-386). In one embodiment, three V_(H) CDR3 residues randomized as described, for example, by Schier et al. (1996) Gene, 169: 147-155; Schier and Marks (1996) Human Antibodies and Hybridomas. 7: 97-105; and Schier et al. (1996) J. Mol. Biol. 263: 551-567.

Other Antibody Modifications.

In one embodiment, partial antibody sequences derived from the anti-CSPG4 antibody (e.g., 9.2.27 or other antibodies in Table 1) may be used to produce structurally and functionally related antibodies. For example, antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions (CDRs). For this reason, the amino acid sequences within CDRs are more diverse between individual antibodies than sequences outside of CDRs. Because CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally occurring antibody grafted onto framework sequences from a different antibody with different properties (see, e.g., Riechmann et al. (1998) Nature 332: 323-327; Jones et al., (1986) Nature 321: 522-525; and Queen et al. (1989) Proc. Natl. Acad. Sci. USA, 86: 10029-10033). Such framework sequences can be obtained from public DNA databases that include germline antibody gene sequences.

Thus, one or more structural features of an anti-CSPG4 antibody described herein (e.g., 9.2.27), such as the CDRs, can be used to create structurally related anti-CSPG4 antibodies that retain at least one functional property of, for example, the 9.2.27 antibody, e.g., binding of target cancer cells.

In a particular embodiment, one or more anti-CSPG4 (e.g., 9.2.27) CDR regions (e.g. VH CDR1, and/or CDR2, and/or CDR3, and/or VL CDR1, and/or CDR2, and/or CDR3) is combined recombinantly with known human framework regions and CDRs to create additional, recombinantly-engineered, anti-CSPG4 antibodies. The heavy and light chain variable framework regions can be derived from the same or different antibody sequences.

It is well known in the art that antibody heavy and light chain CDR3 domains play a particularly important role in the binding specificity/affinity of an antibody for an antigen (see, e.g., Hall et al. (1992) J. Immunol., 149: 1605-1612; Polymenis et al. (1994) J. Immunol., 152: 5318-5329; Jahn et al. (1995) Immunobiol., 193:400-419; Klimka et al. (2000) Brit. J. Cancer, 83: 252-260; Beiboer et al. (2000) J. Mol. Biol, 296: 833-849; Rader et al. (1998) Proc. Natl. Acad. Sci. USA, 95: 8910-8915; Barbas et al. (1994) J. Am. Chem. Soc., 116: 2161-2162; Ditzel et al. (1996) J. Immunol., 157: 739-749). Accordingly, in certain embodiments, antibodies are generated that include the heavy and/or light chain CDR3s of the particular antibodies described herein (e.g., 9.2.27 or other antibodies in Table 1). In certain embodiments, antibodies are generated that include the heavy and/or light chain CDR1s of the particular antibodies described herein (e.g., 9.2.27). The antibodies can further include the other heavy and/or light chain CDRs of the antibodies of the present invention (e.g., 9.2.27 or other antibodies in Table 1).

In certain embodiments the CDR1, 2, and/or 3 regions of the engineered antibodies described above can comprise the exact amino acid sequence(s) as those disclosed herein (e.g., CDRs of 9.2.27 or other antibodies in Table 1). However, the ordinarily skilled artisan will appreciate that some deviation from the exact CDR sequences may be possible while still retaining the ability of the antibody to bind CSPG4 effectively (e.g., conservative amino acid substitutions). Accordingly, in another embodiment, the engineered antibody may be composed of one or more CDRs that are, for example, 90%, 95%, 98%, 99% or 99.5% identical to one or more CDRs of the 9.2.27 or other antibodies in Table 1.

In another embodiment, one or more residues of a CDR may be altered to modify binding to achieve a more favored on-rate of binding. Using this strategy, an antibody having ultra high binding affinity of, for example, 10⁻¹⁰ M or more, can be achieved. Affinity maturation techniques, well known in the art and those described herein, can be used to alter the CDR region(s) followed by screening of the resultant binding molecules for the desired change in binding. Accordingly, as CDR(s) are altered, changes in binding affinity as well as immunogenicity can be monitored and scored such that an antibody optimized for the best combined binding and low immunogenicity are achieved.

In addition to, or instead of, modifications within the CDRs, modifications can also be made within one or more of the framework regions, FR1, FR2, FR3 and FR4, of the heavy and/or the light chain variable regions of an antibody, so long as these modifications do not eliminate the binding affinity of the antibody.

In another embodiment, the antibody is further modified with respect to effector function, so as to enhance the effectiveness of the antibody. For example cysteine residue(s) may be introduced in the Fc region, thereby allowing interchain disulfide bond formation in this region. The homodimeric antibody thus generated may have improved internalization capability and/or increased complement-mediated cell killing and antibody-dependent cellular cytotoxicity (ADCC) (see, e.g., Caron et al. (1992) J. Exp Med. 176: 1191-1195; Shopes (1992) J. Immunol. 148: 2918-2922). Homodimeric antibodies with enhanced anti-tumor activity may also be prepared using heterobifunctional cross-linkers (see, e.g., Wolff et al. (1993) Cancer Res. 53:2560-2565). Alternatively, an antibody can be engineered which has dual Fc regions and may thereby have enhanced complement lysis and ADCC capabilities (see, e.g., Stevenson et al. (1989) Anti-Cancer Drug Design 3: 219-230).

It is noted that in various embodiments, antibodies suitable for use in the antibody-IFN constructs described herein comprise an antibody selected from the group consisting of a full length immunoglobulin, an Fv, an Fab, a (Fab′)₂, a (Fab′)₃, an IgGΔCH2, and a minibody, a unibody, an affibody, and the like. In certain embodiments the antibody is a full length immunoglobulin (e.g., IgA, IgD, IgG or IgM).

Any of the antibodies described herein (e.g., the antibodies in Table 1 such as 9.2.27 and variants thereof, e.g., as described herein) can readily be engineered into any of these formats. In this regard, it is noted that fully human antibodies and fragments thereof that bind to CSPG4 are disclosed in WO 2010/045495 (e.g., an scFv fragment isolated from a semi-synthetic phage display scFv antibody library and designated C21) and similar methods can be used to generate and modify other anti-CSGP4 antibodies. EP 0411893 A2 describes methods and constructs for expressing the 9.2.27 antibody and variants thereof.

With respect to sequence identity described above, it is noted that similarity between amino acid or nucleotide sequences is expressed in terms of the similarity between the sequences, otherwise referred to as sequence identity. Sequence identity is frequently measured in terms of percentage identity (or similarity or homology); the higher the percentage, the more similar the two sequences are. Homologs or variants of the amino acid or nucleotide sequence will possess a relatively high degree of sequence identity when aligned using standard methods.

Methods of alignment of sequences for comparison are well known in the art. Various programs and alignment algorithms are described in: Smith and Waterman, Adv. Appl. Math. 2:482, 1981; Needleman and Wunsch, J. Mol. Biol. 48:443, 1970; Pearson and Lipman, Proc. Natl. Acad. Sci. U.S.A. 85:2444, 1988; Higgins and Sharp, Gene 73:237, 1988; Higgins and Sharp, CABIOS 5:151, 1989; Corpet et al, Nucleic Acids Research 16:10881, 1988; and Pearson and Lipman, Proc. Natl. Acad. Sci. U.S.A. 85:2444, 1988. Altschul et al., Nature Genet. 6:119, 1994, presents a detailed consideration of sequence alignment methods and homology calculations.

The NCBI Basic Local Alignment Search Tool (BLAST) (Altschul et al, J. Mol. Biol. 215:403, 1990) is available from several sources, including the National Center for Biotechnology Information (NCBI, Bethesda, Md.) and on the internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. A description of how to determine sequence identity using this program is available on the NCBI website on the internet.

Homologs and variants of the anti-CSPG4 antibodies or a domain thereof (e.g., a VL, VH, CL or CH domain) typically have at least about 75%, for example at least about 80%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the original sequence (e.g. a sequence defined above), for example counted over the full length alignment with the amino acid sequence of the antibody or domain thereof using the NCBI Blast 2.0, gapped blastp set to default parameters. For comparisons of amino acid sequences of greater than about 30 amino acids, the Blast 2 sequences function can be employed using the default BLOSUM62 matrix set to default parameters, (gap existence cost of 11, and a per residue gap cost of 1). When aligning short peptides (fewer than around 30 amino acids), the alignment can be performed using the Blast 2 sequences function, employing the PAM30 matrix set to default parameters (open gap 9, extension gap 1 penalties). Proteins with even greater similarity to the reference sequences will show increasing percentage identities when assessed by this method, such as at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity. When less than the entire sequence is being compared for sequence identity, homologs and variants will typically possess at least 80% sequence identity over short windows of 10-20 amino acids, and may possess sequence identities of at least 85% or at least 90% or 95% depending on their similarity to the reference sequence. Methods for determining sequence identity over such short windows are available at the NCBI website on the internet. One of skill in the art will appreciate that these sequence identity ranges are provided for guidance only; it is entirely possible that strongly significant homologs could be obtained that fall outside of the ranges provided.

Typically variants may contain one or more conservative amino acid substitutions compared to the original amino acid or nucleic acid sequence. Conservative substitutions are those substitutions that do not substantially affect or decrease the affinity of an antibody to CSPG4. For example, a human antibody that specifically binds CSPG4 may include up to 1, up to 2, up to 5, up to 10, or up to 15 conservative substitutions compared to the original sequence (e.g. as defined above) and retain specific binding to the CSPG4 polypeptide. The term conservative variation also includes the use of a substituted amino acid in place of an unsubstituted parent amino acid, provided that antibody specifically binds CSPG4. Non-conservative substitutions are those that reduce an activity or binding to CSPG4.

Functionally similar amino acids that may be exchanged by way of conservative substitution are well known to one of ordinary skill in the art. The following six groups are examples of amino acids that are considered to be conservative substitutions for one another: 1) Alanine (A), Serine (S), Threonine (T); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); and 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W).

While the above discussion pertains primarily to antibodies, it will be recognized that affybodies and/or unibodies can be used instead of antibodies.

Unibodies.

UniBody are antibody technology that produces a stable, smaller antibody format with an anticipated longer therapeutic window than certain small antibody formats. In certain embodiments unibodies are produced from IgG4 antibodies by eliminating the hinge region of the antibody. Unlike the full size IgG4 antibody, the half molecule fragment is very stable and is termed a uniBody. Halving the IgG4 molecule left only one area on the UniBody that can bind to a target. Methods of producing unibodies are described in detail in PCT Publication WO2007/059782, (see, also, Kolfschoten et al. (2007) Science 317: 1554-1557) and can be used to create unibodies based on any known anti-CSPG4 antibody.

Affibodies.

Affibody molecules are class of affinity proteins based on a 58-amino acid residue protein domain, derived from one of the IgG-binding domains of staphylococcal protein A. This three helix bundle domain has been used as a scaffold for the construction of combinatorial phagemid libraries, from which Affibody variants that target the desired molecules can be selected using phage display technology (see, e.g., Nord et al. (1997) Nat. Biotechnol. 15: 772-777; Ronmark et al. (2002) Eur. J. Biochem., 269: 2647-2655.). Details of Affibodies and methods of production are known to those of skill (see, e.g., U.S. Pat. No. 5,831,).

B) Interferons

In various embodiments chimeric moieties of this invention comprise an interferon (e.g., IFN-α, IFNβ, IFN-γ, etc.) joined to a targeting moiety (e.g., anti-CSPG4 antibody). In various embodiments the interferon can be a full length wild-type interferon (e.g. IFN-α, IFNβ, IFN-γ, etc.) an interferon fragment (e.g., an IFN-α fragment), and/or a mutated interferon. Typically the interferon fragment is one that possesses the endogenous binding affinity and/or activity of the native interferon, preferably at a level of at least 60%, or of at least 80%, more preferably at least 90% or 95%, most preferably at least 98%, 99%, 100%, or a level greater than the wild-type interferon (in its isolated form).

Interferons and interferon mutants are a well known and well characterized group of cytokines (see e.g., WO 2002/095067; WO 2002/079249; WO 2002/101048; WO 2002/095067; WO 2002/083733; WO 2002/086156; WO 2002/083733; WO 2003/000896; WO 2002/101048; WO 2002/079249; WO 2003/000896; WO 2004/022593; WO 2004/022747; WO 2003/023032; WO 2004/022593 and also in Kim et al. (2003) Cancer Lett. 189(2):183-188; Hussain et al. (2000) J. Interferon Cytokine Res. 20(9): 763-768; Hussain et al. (1998) J. Interferon Cytokine Res. 18(7): 469-477; Nyman et al. (1988) Biochem. J. 329 (Pt 2): 295-302; Golovleva et al. (1997) J. Interferon Cytokine Res. 17(10): 637-645; Hussain et al. (1997) J. Interferon Cytokine Res. 17(9): 559-566; Golovleva et al. (1997) Hum. Hered. 47(4): 185-188; Kita et al. (1997) J. Interferon Cytokine Res. 17(3): 135-140; Golovleva et al. (1996) Am. J. Hum. Genet. 59(3): 570-578; Hussain et al. (1996) J. Interferon Cytokine Res. 16(7): 523-529; Linge et al. (1995) Biochim Biophys Acta. 1264(3): 363-368; Gewert et al. (1995) J Interferon Cytokine Res. 15(5): 403-406; Lee et al. (1995) J. Interferon Cytokine Res. 15(4): 341-349; Kaluz et al. (1994) Acta Virol. 38(2): 101-104; Emanuel et al. (1993) J. Interferon Res. 13(3): 227-231; Kaluz et al. (1993) Acta Virol. 37(1): 97-100; Li et al. (1992) Sci. China B. 35(2): 200-206.

By way of illustration, alleles of the human interferon α family of genes/proteins are illustrated in Table 2.

TABLE 2 Common alleles of the human interferon α family of genes/proteins and was constructed based on Pestka (1983) Arch Biochem Biophys 221: 1-37; Diaz et al. (1994) Genomics 22: 540-52; and Pestka (1986) Meth. Enzymol., 119: 3-14; and reviewed in Krause et al. (2000) J. Biol. Chem. 275: 22995-3004. Gene Interferon Proteins (allelic variant names) IFNA1 IFN-α1, IFN- αD IFNA2 IFN- α2, IFN α2b, IFN- αA, IFN-α2a, INF-α2c IFNA4 IFN-α4a, IFNα76, IFN-α4b, IFN-α74, IFN-αM IFNA5 IFN-α5, IFNαG, IFN-α61 IFNA6 IFN-α6, IFN-αK, IFN-α54 IFNA7 IFN-α7, IFN-αJ, IFN-αJ1 IFNA8 IFN-α8, IFN-αB2, IFN-αB IFNA10 IFN-αC, IFN-α61 IFNA13 IFN-α13 IFNA14 IFN-α14, IFN-αH, IFN-αH1 IFNA16 IFN-α16, IFN-αWA, IFN-αO IFNA17 IFN-α17,IFN-α1, IFN-α88 IFNA21 IFN-α21, IFN-αF IFNA22 IFN-α22, IFN-αGX-1

Any of these IFN-α are contemplated for use in the constructs described herein. Additionally IFN-β, IFN-γ, biologically active truncated interferons (truncated IFN-α, IFN-β, IFN-γ), and mutant interferons (e.g., mutant IFN-α, IFN-β, IFN-γ) are contemplated.

In certain embodiments the interferon is a full-length IFN-α, a full-length IFN-β, or a full length IFN-γ.

In certain embodiments the interferon is a biologically active truncated IFN-α, a biologically active truncated IFN-β, or a biologically active truncated IFN-γ.

Means of identifying such truncated or modified interferon molecules are routine to those of skill in the art. In one illustrative approach, a library of truncated and/or mutated IFN-α is produced and screened for IFN-α activity. Methods of producing libraries of polypeptide variants are well known to those of skill in the art. Thus, for example error-prone PCR can be used to create a library of mutant and/or truncated IFN-α (see, e.g., U.S. Pat. No. 6,365,408).

The resulting library members can then be screened according to standard methods know to those of skill in the art. Thus, for example, IFN-α activity can be assayed by measuring antiviral activity against a particular test virus. Kits for assaying for IFN-α activity are commercially available (see, e.g., ILITE™ alphabeta kit by Neutekbio, Ireland).

In various embodiments use of a mutated interferon alpha 2 (IFNα2) is contemplated. Certain mutants include a mutation of the His at position 5, and/or the E at position 5, and/or the Q at position 6. In certain embodiments the mutants include the mutation H57Y, and/or E58N, and/or Q61S. In certain embodiments the mutants include a mutated IFNα2 having the mutations H57Y, E58N, and Q61S (YNS) (see, e.g., Kalie et al. (2007) J. Biol. Chem., 282: 11602-11611).

In other embodiments mutants include a mutation of the His at position 5, and/or the E at position 5, and/or the Q at position 6 to A (alanine). In certain embodiments the mutants include a mutated IFNα2 having the mutations H57A, E58A, and Q61A (HEQ) (see, e.g., Jaitin et al. (2006) Mol. Cellular Biol., 26(5): 1888-1897). In certain embodiments the mutant interferon comprises a mutation of His at position 5 to A, Y, or M, and/or a mutation of E at position 5 to A, or N, or D, or L, and/or a mutation of Q at position 6 to A, or S, or L, or D.

In certain embodiments mutant include mutants of interferon alpha 8 (IFN-α8). Three mutant IFN-α8 molecules were identified (R145V, A146N, M149Y), (R145I, A146S, M149Y), and (R145L, A146S, M149Y)] that displayed improved anti-proliferative activity against a wide range of different cell lines (see, e.g., Yamamoto et. al. (2009) J. Interferon & cytokine Res, 29:161-170. Accordingly, in certain embodiments IFN-α8 mutants are contemplated that have R145 to V, I, or L, and/or A146 to N, or S, and/or M149 to Y are contemplated.

A mutated IFNβ comprising a serine substituted for the naturally occurring cysteine at amino acid 17 has also been demonstrated to show efficacy (see, e.g., Hawkins et al. (1985) Cancer Res., 45, 5914-5920.

In various embodiments use of truncated interferons is also contemplated. Human INFα, for example, with deletions of the first 15 amino-terminal amino acid residues and/or the last 10-13 carboxyl-terminal amino acid residues, have been shown to exhibit virtually the same activity as the parent molecules (see, e.g., Ackerman (1984) Proc. Natl. Acad. Sci., USA, 81: 1045-1047). Accordingly the use of IFN-αs having 1, 2, 3, up to 13 carboxyl terminal amino acid residues deleted and/or 1, 2, 3, up to 15 amino terminal amino acid residues deleted are contemplated.

It has also been demonstrated that activity resides in huIFN-α fragment HuIFN-α (1-110) (Id.). Accordingly carboxyl truncated IFNs with truncations after residue 110 and/or with 1, 2, 3, up to 15 amino terminal amino acid residues deleted are contemplated.

Certain C-terminally truncated interferon betas (IFNβ) have been shown to have increased activity (see, e.g., U.S. Patent Publication 2009/0025106 A1). Accordingly, in certain embodiments the interferon used in the constructs described herein includes the C-terminally truncated IFNβ described as IFN-Δ1, IFN-Δ2, IFN-Δ3, IFN-Δ4, IFN-Δ5, IFN-Δ6, IFN-Δ7, IFN-Δ8, IFN-Δ9, or IFN-Δ10 as described in U.S. Patent Publication NO: 2009/0025106 A1. In certain embodiments the interferon is IFN-Δ7, IFN-Δ8, or IFN-Δ9 (SEQ ID NOs: 57, 59, and 61 in US 2009/0025106 A1 (see, Table 3).

TABLE 3 Truncated IFNβ showing enhanced activity  (see U.S. patent publication 2009/0025106 A1). SEQ Truncated ID IFN Amino Acid Sequence NO IFN-Δ7 Met Gly Lys Met Ala Ser Leu Phe Ala Thr Phe Leu Val Val Leu Val  9 Ser Leu Ser Leu Ala Ser Glu Ser Ser Ala Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln  IFN-Δ8 Met Gly Lys Met Ala Ser Leu Phe Ala Thr Phe Leu Val Val Leu Val 10 Ser Leu Ser Leu Ala Ser Glu Ser Ser Ala Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile the Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr the Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu  IFN-Δ9 Met Gly Lys Met Ala Ser Leu Phe Ala Thr Phe Leu Val Val Leu Val 11 Ser Leu Ser Leu Ala Ser Glu Ser Ser Ala Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile Ser Leu the Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile the Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn

In certain embodiments mutant interferons include, but are not limited to mutant interferons described in U.S. Pat. No. 7,998,469 which is incorporated herein by reference for the mutant interferons described therein. Illustrative and non limiting interferons include, for example, IFNα-2b proteins that have increased resistance proteolysis compared to the unmodified, typically wild-type, protein. The mutant IFNα-2b proteins include those selected from among proteins containing a single amino acid replacement, or a dual amino acid replacement, or a triple amino acid replacement, or 4 amino acid replacements, or 5 amino acid replacement in IFN-α2b:

(SEQ ID NO: 12) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg  Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile  Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile  Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser  Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe  Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr  Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg  Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu  Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn  Leu Gln Glu Ser Leu Arg Ser Lys Glu where the replacements are selected from the group consisting of: L by V at position 3; L by I at position 3; P by S at position 4; P by A at position 4; R by H at position 1; R by Q at position 1; R by H at position 1; R by Q at position 1; M by V at position 1; M by I at position 1; R by H at position 2; R by Q at position 2; R by H at position 2; R by Q at position 2; F by I at position 2; F by V at position 2; L by V at position 3; L by I at position 3; K by Q at position 3; K by T at position 3; R by H at position 3; R by Q at position 3; E by Q at position 4; E by H at position 4; K by Q at position 4; K by T at position 4; E by Q at position 5; E by H at position 5; K by Q at position 7; K by T at position 7; E by Q at position 7; E by H at position 7; K by Q at position 83; K by T at position 83; Y by H at position 8; Y by I at position 8; E by Q at position 9; E by H at position 9; E by Q at position 17; E by H at position 17; P by S at position 19; P by A at position 19; L by V at position 10; L by I at position 10; M by V at position 11; M by I at position 11; E by Q at position 13; E by H at position 13; L by V at position 17; L by I at position 17; R by H at position 10; R by Q at position 10; K by Q at position 11; K by T at position 11; R by H at position 15; R by Q at position 15; L by V at position 18; L by I at position 18; K by Q at position 11; K by T at position 11; E by Q at position 12; E by H at position 12; K by Q at position 13; K by T at position 13; K by Q at position 14; K by T at position 14; Y by H at position 15; Y by I at position 15; P by S at position 17; P by A at position 17; M by V at position 18; M by I at position 18; R by H at position 19; R by Q at position 19; E by Q at position 19; E by H at position 19; L by V at position 11; L by I at position 11; R by H at position 12; R by Q at position 12; K by Q at position 14; K by T at position 14; E by Q at position 15; and E by H at position 15.

In certain embodiments C-terminal deletions of interferon gamma (IFN-γ) are also contemplated (see, e.g., Lundell et al. (1991) Protein Neg., 4(3): 335-341).

In certain embodiments, N-glycosylation sites can be added to increase resistance to proteolysis while maintaining or improving the requisite biological activity. Exemplary N-glycosylation mutants containing duo-amino acid replacements corresponding to the N-X-S or N-X-T consensus sequences are set forth in Example 3. Accordingly, provided herein are IFNα-2b and IFNα-2a mutant proteins having an increased resistance to proteolysis compared to unmodified IFNα-2b and IFNα-2a, selected from among proteins comprising one, or two, or three or four, or five, or more sets of duo-amino acid replacements in IFN-α2b (SEQ ID NO: 12), corresponding to:

D by N at position 2 and P by S at position 4;

D by N at position 2 and P by T at position 4;

L by N at position 3 and Q by S at position 5;

L by N at position 3 and Q by T at position 5;

P by N at position 4 and T by S at position 6;

P by N at position 4 and T by T at position 6;

Q by N at position 5 and H by S at position 7;

Q by N at position 5 and H by T at position 7;

T by N at position 6 and S by S at position 8;

T by N at position 6 and S by T at position 8;

H by N at position 7 and L by S at position 9;

H by N at position 7 and L by T at position 9;

S by N at position 8 and G by S at position 1;

S by N at position 8 and G by T at position 1;

L by N at position 9 and S by S at position 1;

L by N at position 9 and S by T at position 1;

M by N at position 2 and K by S at position 2;

M by N at position 2 and K by T at position 2;

R by N at position 2 and I by S at position 2;

R by N at position 2 and I by T at position 2;

K or R by N at position 2 and S by S at position 2;

K or R by N at position 2 and S by T at position 2;

I by N at position 2 and L by S at position 2;

I by N at position 2 and L by T at position 2;

S by N at position 2 and F by S at position 2;

S by N at position 2 and F by T at position 2;

L by N at position 2 and S by S at position 2;

L by N at position 2 and S by T at position 2;

S by N at position 2 and L by S at position 3;

S by N at position 2 and L by T at position 3;

L by N at position 3 and D by S at position 3;

L by N at position 3 and D by T at position 3;

K by N at position 3 and R by S at position 3;

K by N at position 3 and R by T at position 3;

D by N at position 3 and H by S at position 3;

D by N at position 3 and H by T at position 3;

R by N at position 3 and D by S at position 3;

R by N at position 3 and D by T at position 3;

H by N at position 3 and F by S at position 3;

H by N at position 3 and F by T at position 3;

D by N at position 3 and G by S at position 3;

D by N at position 3 and G by T at position 3;

F by N at position 3 and F by S at position 3;

F by N at position 3 and F by T at position 3;

G by N at position 3 and P by S at position 3;

G by N at position 3 and P by T at position 3;

F by N at position 3 and Q by S at position 4;

F by N at position 3 and Q by T at position 4;

P by N at position 3 and E by S at position 4;

P by N at position 3 and E by T at position 4;

Q by N at position 4 and E by S at position 4;

Q by N at position 4 and E by T at position 4;

E by N at position 4 and F by S at position 43;

E by N at position 4 and F by T at position 43;

E by N at position 4 and G by S at position 4;

E by N at position 4 and G by T at position 4;

F by N at position 43 and N by S at position 4;

F by N at position 43 and N by T at position 4;

G by N at position 4 and Q by S at position 4;

G by N at position 4 and Q by T at position 4;

N by N at position 4 and F by S at position 4;

N by N at position 4 and F by T at position 4;

Q by N at position 4 and Q by S at position 4;

Q by N at position 4 and Q by T at position 4;

F by N at position 4 and K by S at position 4;

F by N at position 4 and K by T at position 4;

Q by N at position 4 and A by S at position 5;

Q by N at position 4 and A by T at position 5;

K by N at position 4 and E by S at position 5;

K by N at position 4 and E by T at position 5;

A by N at position 5 and T by S at position 5;

A by N at position 5 and T by T at position 5;

S by N at position 6 and K by S at position 7;

S by N at position 6 and K by T at position 7;

K by N at position 7 and S by S at position 7;

K by N at position 7 and S by T at position 7;

A by N at position 7 and D by S at position 7;

A by N at position 7 and D by T at position 7;

D by N at position 7 and T by S at position 7;

D by N at position 7 and T by T at position 7;

I by N at position 10 and G by S at position 12;

I by N at position 10 and G by T at position 12;

Q by N at position 11 and V by S at position 13;

Q by N at position 11 and V by T at position 13;

G by N at position 12 and G by S at position 14;

G by N at position 12 and G by T at position 14;

V by N at position 13 and V by S at position 15;

V by N at position 13 and V by T at position 15;

G by N at position 14 and T by S at position 16;

G by N at position 14 and T by T at position 16;

V by N at position 15 and E by S at position 17;

V by N at position 15 and E by T at position 17;

T by N at position 16 and T by S at position 18;

T by N at position 16 and T by T at position 18;

E by N at position 17 and P by S at position 19;

E by N at position 17 and P by T at position 19;

T by N at position 18 and I by S at position 10;

T by N at position 18 and I by T at position 10;

K by N at position 14 and S by S at position 16;

K by N at position 14 and S by T at position 16;

S by N at position 14 and N by S at position 16;

S by N at position 14 and N by T at position 16;

T by N at position 15 and L by S at position 17;

T by N at position 15 and L by T at position 17;

N by N at position 16 and Q by S at position 18;

N by N at position 16 and Q by T at position 18;

L by N at position 17 and E by S at position 19;

L by N at position 17 and E by T at position 19;

Q by N at position 18 and S by S at position 10;

Q by N at position 18 and S by T at position 10;

E by N at position 19 and L by S at position 11;

E by N at position 19 and L by T at position 11;

S by N at position 10 and R by S at position 12;

S by N at position 10 and R by T at position 12;

L by N at position 11 and S by S at position 13;

L by N at position 11 and S by T at position 13;

R by N at position 12 and K by S at position 14;

R by N at position 12 and K by T at position 14;

S by N at position 13 and E by S at position 15; and/or

S by N at position 13 and E by T at position 15,

where residue 1 corresponds to residue 1 of the mature IFNα-2b or IFNα-2a protein set forth in SEQ ID NO:12 or IFN-α2a (CAA23805):

(SEQ ID NO: 13) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg  Arg Thr Leu Met Leu Leu Ala Gln Met Arg Lys Ile  Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln  Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile  Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser  Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe  Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu  Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr  Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg  Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu  Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg  Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn  Leu Gln Glu Ser Leu Arg Ser Lys Glu, respectively. In particular embodiments, the IFNα-2b or IFNα-2a mutant protein has increased resistance to proteolysis compared to unmodified IFNα-2b or IFNα-2a, and is selected from among proteins comprising one, or two, or three, or four, or five or more sets of duo-amino acid replacements in SEQ ID NO:12 corresponding to:

Q by N at position 5 and H by S at position 7;

P by N at position 3 and E by S at position 4;

P by N at position 3 and E by T at position 4;

Q by N at position 4 and E by S at position 4;

Q by N at position 4 and E by T at position 4;

E by N at position 4 and F by S at position 43;

E by N at position 4 and F by T at position 43;

F by N at position 43 and N by S at position 4;

G by N at position 4 and Q by T at position 4;

N by N at position 4 and F by S at position 4;

N by N at position 4 and F by T at position 4;

Q by N at position 4 and Q by S at position 4;

F by N at position 4 and K by S at position 4;

F by N at position 4 and K by T at position 4;

I by N at position 10 and G by S at position 12;

I by N at position 10 and G by T at position 12;

V by N at position 15 and E by S at position 17;

V by N at position 15 and E by T at position 17;

T by N at position 16 and T by S at position 18;

T by N at position 16 and T by T at position 18;

E by N at position 17 and P by S at position 19;

E by N at position 17 and P by T at position 19;

L by N at position 17 and E by S at position 19;

L by N at position 17 and E by T at position 19;

E by N at position 19 and L by S at position 11; and

E by N at position 19 and L by T at position 11.

In certain provided herein are IFNα-2b and IFNα-2a mutant proteins comprising one or more pseudo-wild type mutations at amino acid positions of IFNα-2b or IFNα-2a corresponding to SEQ ID NO:12 or SEQ ID NO: 13. Such pseudo-wild type mutations include 1, or 2, or 3, or 4, or 5, or more mutations at amino acid residues selected from the group consisting of 9, 10, 17, 20, 24, 25, 35, 37, 41, 52, 54, 56, 57, 58, 60, 63, 64, 65, 76, 89, and 90. The mutations can be either one or more of insertions, deletions and/or replacements of the native amino acid residue(s). In one embodiment, the pseudo-wild type replacements are mutations with alanine at each position. In another embodiment, the pseudo-wild type replacements are one or more mutations in SEQ ID NO: 12 corresponding to:

L by A at position 9, L by A at position 1;

Q by A at position 2, I by A at position 2;

S by A at position 2, D by A at position 3;

G by A at position 3, E by A at position 4;

T by A at position 5, P by A at position 5;

L by A at position 5, H by A at position 5;

E by A at position 5, I by A at position 6;

I by A at position 63, F by A at position 6;

N by A at position 6, W by A at position 7, and/or

Y by A at position 8, and Q by A at position 9.

In certain embodiments, the constructs described herein utilize an interferon showing a reduced activity (e.g., a decreased antiviral activity). In certain embodiments such interferons can comprise mutations at amino acid positions of IFNα-2b corresponding, amino acid residues: 2, 7, 8, 11, 13, 15, 16, 23, 26, 28, 29, 30, 31, 32, 33, 53, 69, 91, 93, 98, and/or 101 or to SEQ ID NO:12. Accordingly, in particular embodiments where it is desired to decrease the anti-viral activity of IFNα-2b or IFNα-2a, either one, or two, or three, or 4, or 5 or more of insertions, deletions and/or replacements of the native amino acid residue(s) can be carried out at one or more of amino acid positions of IFNα-2b or IFNα-2a corresponding to SEQ ID NO: 12, amino acid residues: 2, 7, 8, 11, 13, 15, 16, 23, 26, 28, 29, 30, 31, 32, 33, 53, 69, 91, 93, 98, and/or 101.

In certain embodiments, the modified IFNα cytokines are selected from among:

(a) a modified IFNα-2a that is human and is selected from among proteins comprising one, two, three, four, or 5 or more single amino acid replacements in SEQ ID NO:13, corresponding to amino acid positions: 41, 58, 78, 107, 117, 125, 133, and/or 159;

(b) a modified IFNα-c that is human and is selected from among proteins comprising one, two, three, four, or five or more single amino acid replacements in Genbank P01566, sequence:

(SEQ ID NO: 14) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Gly Gln Met Gly Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Arg Ile Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe  Gln Lys Ala Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Glu Asp  Ser Ser Ala Ala Trp Glu Gln Ser Leu Leu Glu Lys  Phe Ser Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu  Thr Pro Leu Met Asn Glu Asp Ser Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Ile  Glu Arg Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Leu Ser Phe Ser Thr  Asn Leu Gln Lys Arg Leu Arg Arg Lys Asp corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(c) a modified IFNα-2c cytokine that is human and is selected from among cytokines comprising one, two, three, four, or five or more amino acid replacements in the sequence:

(SEQ ID NO: 15) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg  Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile  Ser Leu Phe Ser Cys Leu Lys Asp Arg Arg Asp Phe  Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile  Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser  Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe  Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr  Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg  Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu  Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn  Leu Gln Glu Ser Leu Arg Ser Lys Glu corresponding to amino acid positions: 41, 58, 78, 107, 117, 125, 133, and/or 159;

(d) an IFNα-d modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank AAB59403 sequence:

(SEQ ID NO: 16) Cys Asp Leu Pro Glu Thr His Ser Leu Asp Asn Arg  Arg Thr Leu Met Leu Leu Ala Gln Met Ser Arg Ile  Ser Pro Ser Ser Cys Leu Met Asp Arg His Asp Phe  Gly Phe Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe Gln Lys Ala Pro Ala Ile Ser Val Leu His Glu Leu  Ile Gln Gln Ile Phe Asn Leu Phe Thr Thr Lys Asp  Ser Ser Ala Ala Trp Asp Glu Asp Leu Leu Asp Lys  Phe Cys Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Met Gln Glu Glu Arg Val Gly Glu  Thr Pro Leu Met Asn Ala Asp Ser Ile Leu Ala Val  Lys Lys Tyr Phe Arg Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Leu Ser Leu Ser Thr  Asn Leu Gln Glu Arg Leu Arg Arg Lys Glu  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(e) an IFNα-5 modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank CAA26702 sequence:

(SEQ ID NO: 17) Cys Asp Leu Pro Gln Thr His Ser Leu Ser Asn Arg  Arg Thr Leu Met Ile Met Ala Gln Met Gly Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Phe Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe Gln Lys Ala Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Lys Asp  Ser Ser Ala Thr Trp Asp Glu Thr Leu Leu Asp Lys  Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu  Glu Ala Cys Met Met Gln Glu Val Gly Val Glu Asp  Thr Pro Leu Met Asn Val Asp Ser Ile Leu Thr Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Ala  Asn Leu Gln Glu Arg Leu Arg Arg Lys Glu  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(f) an IFNα-6 modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank CAA26704 sequence:

(SEQ ID NO: 18) Cys Asp Leu Pro Gln Thr His Ser Leu Gly His Arg  Arg Thr Met Met Leu Leu Ala Gln Met Arg Arg Ile  Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Arg Phe Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe  Gln Lys Ala Glu Ala Ile Ser Val Leu His Glu Val  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Lys Asp  Ser Ser Val Ala Trp Asp Glu Arg Leu Leu Asp Lys  Leu Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu  Glu Ala Cys Val Met Gln Glu Val Trp Val Gly Gly  Thr Pro Leu Met Asn Glu Asp Ser Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Phe Ser Ser Ser Arg  Asn Leu Gln Glu Arg Leu Arg Arg Lys Glu corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(g) an IFNα-4 modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank NP_066546 sequence:

(SEQ ID NO: 19) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Gly Arg Ile  Ser His Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Phe Pro Glu Glu Glu Phe Asp Gly His Gln Phe  Gln Lys Ala Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Glu Asp  Ser Ser Ala Ala Trp Glu Gln Ser Leu Leu Glu Lys  Phe Ser Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu  Thr Pro Leu Met Asn Glu Asp Ser Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Leu Ser Phe Ser Thr  Asn Leu Gln Lys Arg Leu Arg Arg Lys Asp  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(h) an IFNα-4b modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank CAA26701 sequence:

(SEQ ID NO: 20) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Gly Arg Ile  Ser His Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Phe Pro Glu Glu Glu Phe Asp Gly His Gln Phe  Gln Lys Thr Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Glu Asp  Ser Ser Ala Ala Trp Glu Gln Ser Leu Leu Glu Lys  Phe Ser Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu  Thr Pro Leu Met Asn Val Asp Ser Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Leu Ser Phe Ser Thr  Asn Leu Gln Lys Arg Leu Arg Arg Lys Asp corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(i) the IFNα-I modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank AAA52725 sequence:

(SEQ ID NO: 21) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Gly Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Leu Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe  Gln Lys Thr Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Glu Asp  Ser Ser Ala Ala Trp Glu Gln Ser Leu Leu Glu Lys  Phe Ser Thr Glu Leu Tyr Gln Gln Leu Asn Asn Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Met Glu Glu  Thr Pro Leu Met Asn Glu Asp Ser Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Leu Ser Phe Ser Thr  Asn Leu Gln Lys Ile Leu Arg Arg Lys Asp corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(j) an IFNα-J modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank CAA23792 sequence:

(SEQ ID NO: 22) Cys Asp Leu Pro Gln Thr His Ser Leu Arg Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Gly Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Glu Phe  Arg Phe Pro Glu Glu Glu Phe Asp Gly His Gln Phe  Gln Lys Thr Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Glu Asp  Ser Ser Ala Ala Trp Glu Gln Ser Leu Leu Glu Lys  Phe Ser Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu  Thr Pro Leu Met Asn Glu Asp Phe Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Met  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Phe Ser Phe Ser Thr  Asn Leu Lys Lys Gly Leu Arg Arg Lys Asp  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(k) an IFNα-H modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank CAA23794 sequence:

(SEQ ID NO: 23) Cys Asn Leu Ser Gln Thr His Ser Leu Asn Asn Arg  Arg Thr Leu Met Leu Met Ala Gln Met Arg Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Glu Phe Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe  Gln Lys Ala Gln Ala Ile Ser Val Leu His Glu Met  Met Gln Gln Thr Phe Asn Leu Phe Ser Thr Lys Asn  Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Glu Lys  Phe Tyr Ile Glu Leu Phe Gln Gln Met Asn Asp Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu  Thr Pro Leu Met Asn Glu Asp Ser Ile Leu Ala Val  Lys Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Met  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Leu Ser Phe Ser Thr  Asn Leu Gln Lys Arg Leu Arg Arg Lys Asp  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(1) an IFNα-F modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank AAA52718 sequence:

(SEQ ID NO: 24) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Gly Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Phe Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe  Gln Lys Ala Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Lys Asp  Ser Ser Ala Thr Trp Glu Gln Ser Leu Leu Glu Lys  Phe Ser Thr Glu Leu Asn Gln Gln Leu Asn Asp Leu  Glu Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu  Thr Pro Leu Met Asn Val Asp Ser Ile Leu Ala Val  Lys Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Lys  Ile Phe Gln Glu Arg Leu Arg Arg Lys Glu  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160;

(m) an IFNα-8 modified protein that is human and is selected from among proteins comprising one, or two, or three, or four, or five or more single amino acid replacements in Genbank CAA26903, sequence:

(SEQ ID NO: 25) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Arg Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Glu Phe Pro Gln Glu Glu Phe Asp Asp Lys Gln Phe  Gln Lys Ala Gln Ala Ile Ser Val Leu His Glu Met  Ile Gln Gln Thr Phe Asn Leu Phe Ser Thr Lys Asp  Ser Ser Ala Ala Leu Asp Glu Thr Leu Leu Asp Glu  Phe Tyr Ile Glu Leu Asp Gln Gln Leu Asn Asp Leu  Glu Ser Cys Val Met Gln Glu Val Gly Val Ile Glu  Ser Pro Leu Met Tyr Glu Asp Ser Ile Leu Ala Val  Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr  Glu Lys Lys Tyr Ser Ser Cys Ala Trp Glu Val Val  Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Ile  Asn Leu Gln Lys Arg Leu Lys Ser Lys Glu  corresponding to amino acid positions: 41, 59, 79, 108, 118, 126, 134, and/or 160; and/or

(n) an IFNα-consensus modified protein sequence that is human and is selected from among proteins that contain one, or two, or three, or four, or five or more single amino acid replacements in the consensus sequence:

(SEQ ID NO: 26) Cys Asp Leu Pro Gln Thr His Ser Leu Gly Asn Arg  Arg Ala Leu Ile Leu Leu Ala Gln Met Arg Arg Ile  Ser Pro Phe Ser Cys Leu Lys Asp Arg His Asp Phe  Gly Pro Gln Glu Glu Phe Asp Gly Asn Gln Phe Gln  Lys Ala Gln Ala Ile Ser Val Leu His Glu Met Ile  Gln Gln Thr Phe Asn Leu Phe Ser Thr Lys Asp Ser  Ser Ala Ala Trp Asp Glu Ser Leu Leu Glu Lys Phe  Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu  Ala Cys Val Ile Gln Glu Val Gly Val Glu Glu Thr  Pro Leu Met Asn Val Asp Ser Ile Leu Ala Val Lys  Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Thr Glu  Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg  Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn  Leu Gln Glu Arg Leu Arg Arg Lys Glu corresponding to amino acid positions: 41, 58, 78, 107, 117, 125, 133, and/or 159.

Also contemplated are modified IFNβ cytokines, comprising mutations at one, or two, or three, or four, or five or more amino acid residues of IFNβ (GENBANK AAC41702) sequence:

(SEQ ID NO: 27) Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser  Ser Asn Phe Gln Cys Gln Lys Leu Leu Trp Gln Leu  Asn Gly Arg Leu Glu Tyr Cys Leu Lys Asp Arg Met  Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln  Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr  Glu Met Leu Gln Asn Ile Phe Ala Ile Phe Arg Gln  Asp Ser Ser Ser Thr Gly Trp Asn Glu Thr Ile Val  Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn  His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys  Glu Asp Phe Thr Arg Gly Lys Leu Met Ser Ser Leu  His Leu Lys Arg Tyr Tyr Gly Arg Ile Leu His Tyr  Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr  Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe  Ile Asn Arg Leu Thr Gly Tyr Leu Arg Asn  corresponding to one, two, three, four, or five or more positions selected from the group consisting of position 16: 39, 42, 45, 47, 52, 67, 71, 73, 81, 107, 108, 109, 110, 111, 113, 116, 120, 123, 124, 128, 130, 134, 136, 137, 163, and 165. The mutations include insertions, deletions and replacements of the native amino acid residue(s). In particular embodiments, the replacements are selected from among amino acid substitutions in SEQ ID NO:27 set forth in FIG. 12A of U.S. Pat. No. 7,998,469 B2, where the first amino acid indicated is substituted by the second at the position indicated for all of the substitutions set forth in FIG. 12A through 12T of U.S. Pat. No. 7,998,469 B2 which is incorporated herein by reference for the substitutions set for in FIGS. 12a through 12T therein.

In various embodiments proteinase resistant modified interferon-beta polypeptides as described in U.S. Pat. No. 8,052,964 B2 are also contemplated. Certain illustrative modified IFN-β molecules differ from an unmodified IFN beta by two amino acid substitutions where the unmodified IFN beta cytokine comprises the amino acid sequence of IFN-β (Genbank AAC41702, SEQ ID NO:27) and the two amino acid substitutions are selected from the group consisting of substitution of the 5th and 6th positions in SEQ ID NO:27, with aspartic acid and glutamine, respectively; or substitution of the 5th and 6th positions in SEQ ID NO:27, with glutamine; or substitution of the 5th and 6th positions in SEQ ID NO:27, with asparagine and glutamine, respectively; or substitution of the 6th and 36th positions in SEQ ID NO:27, with glutamine and isoleucine, respectively; or substitution of the 6th and 86th positions in SEQ ID NO:27, with glutamine where the two amino acid substitutions confer increased resistance to proteolysis over the unmodified IFN beta.

In certain embodiments the use of an interferon gamma (designated IFNγ or IFN-γ), a truncated IFN-γ, or a mutant IFN-γ is contemplated.

Interferon-gamma (IFNγ) is a cytokine produced by T-lymphocytes and natural killer cells and exists as a homodimer of two noncovalently bound polypeptide subunits. The mature form of each dimer comprises 143 amino acid residues (shown in SEQ ID NO:28):

(SEQ ID NO: 28) DPYVKEAENL KKYFNAGHSD VADNGTLFLG ILKNWKEESD  RKIMQSQIVS FYFKLFKNFK DDQSIQKSVE TIKEDMNVKF  FNSNKKKRDDF EKLTNYSVT DLNVQRKAIH ELIQVMAELS  PAAKTGKRKR SQMLFQGRRAS Q 

Each subunit has two potential N-glycosylation sites (Aggarwal et al. (1992) Human Cytokines, Blackwell Scientific Publications) at positions 25 and 97. Depending on the degree of glycosylation the molecular weight of IFNG in dimer form is 34-50 kDa (Farrar et al. (1993) Ann. Rev. Immunol, 11: 571-611).

The primary sequence of wildtype human IFNG (huIFNγG) was reported by Gray et al. (1982) Nature 298: 859-863), Taya et al. (1982) EMBO J. 1: 953-958; Devos et al. (1982) Nucleic Acids Res. 10: 2487-2501; and Rinderknecht et al. (1984) J. Biol. Chem. 259: 6790-6797), and in EP 77670, EP 89676 and EP 110044. The 3D structure of huIFNG was reported by Ealick et al. (1991) Science 252: 698-702, 1991).

Various naturally-occurring or mutated forms of the IFNG subunit polypeptides have been reported, including one comprising a Cys-Tyr-Cys N-terminal amino acid sequence (positions (−3)-(−1) relative to SEQ ID NO:28), one comprising an N-terminal methionine (position −1 relative to SEQ ID NO:28), and various C-terminally truncated forms comprising 127-134 amino acid residues. It is known that 1-15 amino acid residues may be deleted from the C-terminus without abolishing IFNγ activity of the molecule. Furthermore, heterogeneity of the huIFNγ C-terminus was described by Pan et al. (1987) Eur. J. Biochem. 166: 145-149.

HuIFNγ muteins were reported by Slodowski et al. (1991) Eur. J. Biochem. 202:1133-1140, 1991, Luk et al. (1990) J. Biol. Chem. 265: 13314-13319, Seelig et al., (1988) Biochemistry 27: 1981-1987, Trousdale et al. (1985) Invest. Ophthalmol. Vis. Sci. 26: 1244-1251, and in EP 146354.

WO 1992/008737 discloses IFNγ variants comprising an added methionine in the N-terminal end of the full (residues 1-143) or partial (residues 1-132) amino acid sequence of wildtype human IFNG. EP 219 781 discloses partial huIFNγ sequences comprising amino 10 acid residues 3-124 (of SEQ ID NO:28)). U.S. Pat. No. 4,832,959 discloses partial huIFNG sequences comprising residues 1-127, 5-146 and 5-127 of an amino acid sequence that compared to SEQ ID NO 2 has three additional N-terminal amino acid residues (CYC). U.S. Pat. No. 5,004,689 discloses a DNA sequence encoding huIFNG without the 3 N-terminal amino acid residues CYC and its expression in E. coli. EP 446582 discloses E. coli produced rhuIFNG free of an 15 N-terminal methionine. U.S. Pat. No. 6,120,762 discloses a peptide fragment of huIFNγ comprising residues 95-134 thereof (relative to SEQ ID NO:28).

In various embodiments where interferon gamma is utilized in the constructs described herein the interferon gamma component(s) of the construct can be any polypeptide with IFNγ activity, and thus be derived from any origin, e.g. a non-human mammalian origin. However, in various embodiments, it is preferred that the parent polypeptide is huIFNγ, e.g., with the amino acid sequence shown in SEQ ID NO:28, or a variant or fragment thereof.

Examples of variants of hIFNγ that can be incorporated in the constructs contemplated herein described above, and include, but are not limited to, e.g. huIFNγ with the N-terminal addition CYC, the cysteine modified variants described in U.S. Pat. No. 6,046,034, and the like. Specific examples of fragments are those described above, and include, but are not limited to huIFNγ C-terminally truncated with 1-15 amino acid residues, e.g. with 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 amino acid residues, and/or N-terminally truncated with 1-3 amino acid residues. In one illustrative, but non-limiting embodiment, the interferon comprises a truncated interferon consisting of the amino acid sequence:

(SEQ ID NO: 29) DPYVKEAENL KKYFNAGHSD VADNGTLFLG ILKNWKEESD  RKIMQSQIVS FYFKLFKNFK DDQSIQKSVE TIKEDMNVKF  FNSNKKKRDD FEKLTNYSVT DLNVQRKAIH ELIQVMAELS  PAAKTGKRKR SQM 

In certain embodiments the use of chemically modified interferon is also contemplated. For example, in certain embodiments, the interferon is chemically modified to increase serum half-life. Thus, for example, (2-sulfo-9-fluorenylmethoxycarbonyl)₇-interferon-α2 undergoes time-dependent spontaneous hydrolysis, generating active interferon (see, e.g., Shechter et al. (2001) Proc. Natl. Acad. Sci., USA, 98(3): 1212-1217). Other modifications, include for example, N-terminal modifications in including, but not limited to the addition of PEG, protecting groups, and the like. U.S. Pat. No. 5,824,784, for example, described N-terminally chemically modified interferon.

TABLE 4 Illustrative, but non-limiting antibody-interferon combinations contemplated for use in the constructs described herein. Where the antibody is listed, the native antibody, chimeric antibodies, humanized antibodies, single chain antibodies are contemplated in addition to full-length immunoglobulins (e.g., IgE, IgG, IgM, IgA, etc.). Interferon Attached Antibody IFN-α1 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α1 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α13 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α14 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α16 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α17 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α2 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α21 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α22 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α2a 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α2b 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α4a 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α4b 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α5 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α54 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α6 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α61 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α61 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α7 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α74 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFNα76 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α8 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-α88 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αA 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αB 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αB2 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αC 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αD 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αF 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αG 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αGX-1 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αH 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αH1 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αJ 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αJ1 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αK 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αM 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αO 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-αWA 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 INF-α2c 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-β 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-γ 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-Δ7 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-Δ8 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 IFN-Δ9 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 TP41.2 mutated IFNα2 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 having the TP41.2 mutations H57A, and/or E58A, and/or Q61A (HEQ) mutated IFNβ 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 comprising a TP41.2 serine substituted for the naturally occurring cysteine at amino acid 17 INFα with 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 deletions of the TP41.2 first 15 amino- terminal amino acid residues and/or the last 10-13 carboxyl- terminal amino acid residues Interferon-α 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 subtype α8 TP41.2 represented by any one of SEQ ID NOs: 1 to 3 in EP 1842857 B1, where the arginine residue at position 145 is replaced with leucine, isoleucine, or valine; and/or alanine residue at position 146 is replaced with asparagine or serine; and/or methionine at position 149 is replaced with tyrosine. Interferon-α 9.2.27 225.28 225.28s 763.74 VF1- VT80.1 653.25 763.74 TP61.5 subtype α8 TP41.2 mutant in EP 1842857 B1, where either of the lysine residues position 31 and/or 134 is retained but the remaining lysine residues are replaced with other amino acid residue (s) while retaining an anti- viral and/or cell- proliferation inhibitory activity. IFN-α1 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL- TP34 TP41.2 14 IFN-α1 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α13 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α14 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α16 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α17 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α2 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α21 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α22 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α2a T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α2b T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α4a T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α4b T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α5 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α54 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α6 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α61 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α61 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α7 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α74 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFNα76 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α8 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-α88 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αA T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αB T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αB2 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αC T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αD T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αF T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αG T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αGX-1 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αH T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αH1 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αJ T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αJ1 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αK T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αM T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αO T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-αWA T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 INF-α2c T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-β T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-γ T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-Δ7 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-Δ8 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 IFN-Δ9 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 TP34 TP41.2 14 mutated IFNα2 T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 having the TP34 TP41.2 14 mutations H57A, and/or E58A, and/or Q61A (HEQ) mutated IFNβ T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 comprising a TP34 TP41.2 14 serine substituted for the naturally occurring cysteine at amino acid 17 INFα with T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 deletions of the TP34 TP41.2 14 first 15 amino- terminal amino acid residues and/or the last 10-13 carboxyl- terminal amino acid residues Interferon-α T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 subtype α8 TP34 TP41.2 14 represented by any one of SEQ ID NOs: 1 to 3 in EP 1842857 B1, where the arginine residue at position 145 is replaced with leucine, isoleucine, or valine; and/or alanine residue at position 146 is replaced with asparagine or serine; and/or methionine at position 149 is replaced with tyrosine. Interferon-α T8-203 149.53 225.28 763.74 TP61.5 VF1- VF1- MEL- MEL-5 subtype α8 TP34 TP41.2 14 mutant in EP 1842857 B1, where either of the lysine residues position 31 and/or 134 is retained but the remaining lysine residues are replaced with other amino acid residue (s) while retaining an anti- viral and/or cell- proliferation inhibitory activity.

The foregoing interferons and interferon/Ab constructs are intended to be illustrative and not limiting. Using the teaching provided herein, other suitable modified interferons (e.g., modified IFN-α, IFNβ, IFN-γ, etc.) and constructs can readily be identified and produced.

C. Attachment of the Targeting Moiety (e.g., Anti-CSPG4 Antibody) to the Interferon.

In various embodiments, the targeting moiety (e.g., an anti-CSPG4 antibody) and the interferon can be joined together in any order. Thus, for example, the antibody can be joined to either the amino or carboxy terminal of the interferon. The antibody can also be joined to an internal region of the interferon, or conversely, the interferon can be joined to an internal location or to any terminus of the antibody, as long as the attachment does not interfere with binding of the antibody to that target marker (e.g., CSPG4).

The antibody and the interferon (e.g., IFN-α, IFNβ, etc.) can be attached by any of a number of means well known to those of skill in the art. In certain embodiments, the interferon is conjugated, either directly or through a linker (spacer), to the antibody. In certain embodiments, however, it is preferable to recombinantly express the construct as a fusion protein (e.g., with a single chain antibody, or with one chain of a multi-chain antibody).

i) Chemical Conjugation of the Targeting Moiety to the Interferon.

In certain embodiments, the targeting moiety (e.g., an anti-CSPG4 antibody) is chemically conjugated to the interferon (e.g., IFN-α, IFNβ, mutIFNα, etc.) molecule. Means of chemically conjugating molecules are well known to those of skill.

The procedure for conjugating two molecules varies according to the chemical structure of the agent. Polypeptides typically contain variety of functional groups; e.g., carboxylic acid (COOH) or free amine (—NH₂) groups that are available for reaction with a suitable functional group on the other peptide, or on a linker to join the molecules thereto.

Alternatively, the antibody and/or the IFN-α can be derivatized to expose or attach additional reactive functional groups. The derivatization can involve attachment of any of a number of linker molecules such as those available from Pierce Chemical Company, Rockford Ill.

A “linker”, as used herein, typically refers to a molecule that is used to join the antibody to the interferon. In various embodiments, the linker is capable of forming covalent bonds to both the antibody and to the interferon. Suitable linkers are well known to those of skill in the art and include, but are not limited to, straight or branched-chain carbon linkers, heterocyclic carbon linkers, or peptide linkers. In certain embodiments, the linker(s) can be joined to the constituent amino acids of the antibody and/or the interferon through their side groups (e.g., through a disulfide linkage to cysteine). In certain preferred embodiments, the linkers are joined to the alpha carbon amino and/or carboxyl groups of the terminal amino acids of the antibody and/or the interferon.

A bifunctional linker having one functional group reactive with a group on the antibody and another group reactive on the interferon, can be used to form the desired conjugate. Alternatively, derivatization can involve chemical treatment of the targeting moiety. Procedures for generation of, for example, free sulfhydryl groups on polypeptides, such as antibodies or antibody fragments, are known (See U.S. Pat. No. 4,659,839).

Many procedures and linker molecules for attachment of various compounds including radionuclide metal chelates, toxins and drugs to proteins such as antibodies are known. See, for example, European Patent Application No. 188,256; U.S. Pat. Nos. 4,671,958, 4,659,839, 4,414,148, 4,699,784; 4,680,338; 4,569,789; and 4,589,071; and Borlinghaus et al. (1987) Cancer Res. 47: 4071-4075. In particular, production of various immunotoxins is well-known within the art and can be found, for example in “Monoclonal Antibody-Toxin Conjugates: Aiming the Magic Bullet,” Thorpe et al., Monoclonal Antibodies in Clinical Medicine, Academic Press, pp. 168-190 (1982); Waldmann (1991) Science, 252: 1657; U.S. Pat. Nos. 4,545,985 and 4,894,443, and the like.

ii) Production of Fusion Proteins.

In certain embodiments, a chimeric targeting moiety-interferon fusion protein is synthesized using recombinant DNA methodology. Generally this involves creating a DNA sequence that encodes the fusion protein, placing the DNA in an expression cassette under the control of a particular promoter, expressing the protein in a host, isolating the expressed protein and, if required, renaturing the protein.

DNA encoding the fusion proteins or encoding one chain of the antibody attached to an interferon can be prepared by any suitable method, including, for example, cloning and restriction of appropriate sequences or direct chemical synthesis by methods such as the phosphotriester method of Narang et al. (1979) Meth. Enzymol. 68: 90-99; the phosphodiester method of Brown et al. (1979) Meth. Enzymol. 68: 109-151; the diethylphosphoramidite method of Beaucage et al. (1981) Tetra. Lett., 22: 1859-1862); the solid support method of U.S. Pat. No. 4,458,066, and the like.

Chemical synthesis produces a single stranded oligonucleotide. This can be converted into double stranded DNA by hybridization with a complementary sequence, or by polymerization with a DNA polymerase using the single strand as a template. One of skill would recognize that while chemical synthesis of DNA is limited to sequences of about 300 bases, longer sequences may be obtained by the ligation of shorter sequences.

Alternatively, subsequences can be cloned and the appropriate subsequences cleaved using appropriate restriction enzymes. The fragments can then be ligated to produce the desired DNA sequence.

In certain embodiments, DNA encoding fusion proteins can be cloned using DNA amplification methods such as polymerase chain reaction (PCR). Thus, for example, the gene for IFN-α is PCR amplified, using a sense primer containing the restriction site for, e.g., NdeI and an antisense primer containing the restriction site for HindIII. This can produce a nucleic acid encoding the mature IFN-α sequence and having terminal restriction sites. An antibody having “complementary” restriction sites can similarly be cloned and then ligated to the IFN-α and/or to a linker attached to the IFN-α. Ligation of the nucleic acid sequences and insertion into a vector produces a vector encoding IFN-α joined to the anti-CSPG4 antibody.

While the two molecules can be directly joined together, one of skill will appreciate that the molecules can be separated by a peptide spacer consisting of one or more amino acids. Generally the spacer will have no specific biological activity other than to join the proteins or to preserve some minimum distance or other spatial relationship between them. In certain embodiments, however, the constituent amino acids of the spacer can be selected to influence some property of the molecule such as the folding, net charge, or hydrophobicity.

It was a surprising discovery, however, that certain linkers are unsuitable or less preferred for preparation of fusion proteins described herein. Thus, for example, the (Gly₄Ser)₃ (SEQ ID NO: 1) linker was not well suited for the production of certain antibody IFN-α constructs. Without being bound to a particular theory, it is believed the interferon was being removed from the fusion protein by proteolysis. Western blot analysis using anti-Fc and anti-interferon, confirmed that both of the upper bands were heavy chains, but only the largest contained interferon.

Accordingly, in certain preferred embodiments, it is desirable to use a linker that is resistant to proteolysis. Certain preferred linkers are linkers that are not or that do not comprise the (Gly₄Ser)₃ (SEQ ID NO:30) linker. Certain preferred linkers are linkers shorter than 15 amino acids, or linkers shorter than 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2 amino acids in length. In certain embodiments the linker is an alpha helical linker ranging in length up to about 12 or 13 or 14 amino acids in length.

Certain illustrative proteolysis-resistant linkers well suited for use in the constructs of this invention are shown in Table 5.

TABLE 5  Illustrative proteolysis-resistant linkers. Linker Seq SEQ ID NO GGG GGS GGGGS 31 SGGGGS 32 GGGGSGGGGS 33 A EAAAK A 34 A EAAAK EAAAK A 35 A EAAAK EAAAK EAAAK A 36 A EAAAK EAAAK EAAAK EAAAK A 37 A EAAAK EAAAK EAAAK EAAAK EAAAK A 38 AEAAAKEAAAKAG 39 AEAAAKEAAAKAGS 40 GGGGG 41 GGAGG 42 GGGGGGGG 43 GAGAGAGAGA 44 RPLSYRPPFPFGFPSVRP 45 YPRSIYIRRRHPSPSLTT 46 TPSHLSHILPSFGLPTFN 47 RPVSPFTFPRLSNSWLPA 48 SPAAHFPRSIPRPGPIRT 49 APGPSAPSHRSLPSRAFG 50 PRNSIHFLHPLLVAPLGA 51 MPSLSGVLQVRYLSPPDL 52 SPQYPSPLTLTLPPHPSL 53 NPSLNPPSYLHRAPSRIS 54 LPWRTSLLPSLPLRRRP 55 PPLFAKGPVGLLSRSFPP 56 VPPAPVVSLRSAHARPPY 57 LRPTPPRVRSYTCCPTP 58 PNVAHVLPLLTVPWDNLR 59 CNPLLPLCARSPAVRTFP 60 LGTPTPTPTPTGEF 61 EDFTRGKL 62 L EAAAR EAAAR EAAAR EAAAR 63 L EAAAR EAAAR EAAAR 64 L EAAAR EAAAR 65 L EAAAR 66 EAAAR EAAAR EAAAR EAAAR 67 EAAAR EAAAR EAAAR 68 EAAAR EAAAR 69 EAAAR 70 LTEEQQEGGG 71 TEEQQEGGG 72 LAKLKQKTEQLQDRIAGGG 73 LELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCP 74 EPKSCDTPPPCPRCPEPKSCDTPPPCPRCPGG LEPKSSDKTHTSPPSPGG 75

The nucleic acid sequences encoding the fusion proteins can be expressed in a variety of host cells, including E. coli, other bacterial hosts, yeast, and various higher eukaryotic cells such as the COS, CHO and HeLa cells lines and myeloma cell lines. The recombinant protein gene is typically operably linked to appropriate expression control sequences for each host. For E. coli this includes a promoter such as the T7, trp, or lambda promoters, a ribosome binding site and preferably a transcription termination signal. For eukaryotic cells, the control sequences will include a promoter and preferably an enhancer derived from immunoglobulin genes, SV40, cytomegalovirus, etc., and a polyadenylation sequence, and may include splice donor and acceptor sequences.

The plasmids of the invention can be transferred into the chosen host cell by well-known methods such as calcium chloride transformation for E. coli and calcium phosphate treatment or electroporation for mammalian cells. Cells transformed by the plasmids can be selected by resistance to antibiotics conferred by genes contained on the plasmids, such as the amp, gpt, neo and hyg genes.

Once expressed, the recombinant fusion proteins can be purified according to standard procedures of the art, including ammonium sulfate precipitation, affinity columns, column chromatography, gel electrophoresis and the like (see, generally, R. Scopes (1982) Protein Purification, Springer-Verlag, N.Y.: Deutscher (1990) Methods in Enzymology Vol. 182: Guide to Protein Purification, Academic Press, Inc. N.Y., and the like). Substantially pure compositions of at least about 90 to 95% homogeneity are preferred, and 98 to 99% or more homogeneity are most preferred for pharmaceutical uses. Once purified, partially or to homogeneity as desired, the polypeptides may then be used therapeutically.

One of skill in the art would recognize that after chemical synthesis, biological expression, or purification, the fusion protein (e.g., anti-CSPG4-IFN-α, anti-CSPG4-mutIFN-α, etc.) may possess a conformation substantially different than the native conformations of the constituent polypeptides. In this case, it may be necessary to denature and reduce the polypeptide and then to cause the polypeptide to re-fold into the preferred conformation. Methods of reducing and denaturing proteins and inducing re-folding are well known to those of skill in the art (see, e.g., Debinski et al. (1993) J. Biol. Chem., 268: 14065-14070; Kreitman and Pastan (1993) Bioconjug. Chem., 4: 581-585; and Buchner, et al. (1992) Anal. Biochem., 205: 263-270). Debinski et al., for example, describe the denaturation and reduction of inclusion body proteins in guanidine-DTE. The protein is then refolded in a redox buffer containing oxidized glutathione and L-arginine.

In certain embodiments a transient expression system can be used to express the chimeric constructs described herein. Although many cell lines potentially can be used, one cell line that works well for transient expression is 293T. For transient expression of 293T on Day 0, 9 million cells in 25 ml are seeded for each 150 mm tissue culture plate. A 1 mg/ml of PEI (Polyethylenimine) is made using sterile water. For the expression of a complete antibody or antibody fusion protein, 25 μg each of H and L (50 ug total) is used per plate. A volume of 5 ml is used for transfection of each 150 mm plate. The DNA is mixed with DMEM, the PEI is then added and the mixture is incubated at room temperature for 10 mins. 1.75 μg PEI is used for each ug of DNA. For transfection, the old medium is removed, discarded and replaced with 20 ml of fresh medium (Iscoves+5% calf serum). The transfection mix is added and the plate is swirled. On Day 2, the medium is replaced with 30 ml of Iscoves medium containing 1% FBS (fetal bovine serum) to minimize the amount of bovine Ig present. Supernatants are collected from the cells on Days 4, 6 and 13 by removing the medium and replacing it with 30 ml of fresh Iscover containing 1% FBS.

One of skill would recognize these expression methods are illustrative and not limiting. Modifications can be made to the fusion proteins described herein without diminishing their activity/efficacy. Some modifications may be made to facilitate the cloning, expression, or incorporation of the targeting molecule into a fusion protein. Such modifications are well known to those of skill in the art and include, for example, a methionine added at the amino terminus to provide an initiation site, or additional amino acids placed on either terminus to create conveniently located restriction sites or termination codons.

Other modifications can be made to increase serum half-life and/or bioavailability. Such modifications include, but are not limited to the incorporation of D amino acids (especially in the linker), the use of non-naturally occurring amino acids, pegylation of the fusion protein, and the like.

D. Other Multi-Valent Targeting Moieties.

In certain embodiments this invention contemplates the use of multivalent, preferably trivalent, quadravalent, pentavalent or greater targeting moieties to target the interferon to a target cell.

For example, multivalent anti-CSPG4 moieties can be produced by any of a number of methods. For example, linkers having three, four, or more reactive sites can be reacted with anti-CSPG4 antibodies to form a trimer or greater conjugate.

In certain embodiments, phage display, yeast display, bacterial display, or other display systems can be used to express and display multiple copies (e.g., at least 3, at least 4, at least 5, at least 6 copies, etc.) of a targeting antibody (e.g., anti-CSPG4 such as B-B4) and thereby effectively provide a multivalent targeting moiety.

In certain embodiments the use of diabodies and triabodies (e.g., comprising two domains that bind CSPG4 or one domain that binds CSPG4 and another domain that binds, for example, a member of the EGFR receptor family (e.g., EGFR, HER3, etc.). Typically, diabodies comprise a heavy (VH) chain variable domain connected to a light chain variable domain (VL) on the same polypeptide chain (VH-VL) connected by a peptide linker that is too short to allow pairing between the two domains on the same chain. This forces pairing with the complementary domains of another chain and promotes the assembly of a dimeric molecule with two functional antigen binding sites (see, e.g., Holliger et al. (1993) Proc. Natl. Acad. Sci., 90: 6444-6448). In certain embodiments to construct bispecific diabodies the V-domains of antibody A and antibody B are fused to create the two chains VHA-VLB, VHB-VLA. Each chain is inactive in binding to antigen, but recreates the functional antigen binding sites of antibodies A and B on pairing with the other chain.

II. Combined Uses.

The constructs described herein are useful for inhibiting the growth and/or proliferation of target cells (e.g., cancer cells). In various embodiments the constructs can be used to inhibit disease progression, to reduce the rate of secondary tumor formation, to shrink tumor size, and/or to stabilize regression/remission.

Particularly in the treatment of cancer, the constructs, formulations, and methods described herein may also include additional therapeutic and/or pharmacologically acceptable agents. For instance, the constructs, formulations, or methods may involve other agents for the treatment of cancer. Such agents include, but are not limited to alkylating agents (e.g., mechlorethamine (MUSTARGEN®), cyclophosphamide (CYTOXAN®, NEOSAR®)), ifosfamide (IFEX®), phenylalanine mustard; melphalen (ALKERAN®), chlorambucol (LEUKERAN®), uracil mustard, estramustine (EMCYT®), thiotepa (THIOPLEX®), busulfan (MYERLAN®), lomustine (CEENU®), carmustine (BICNU®, BCNU®), streptozocin (ZANOSAR®), dacarbazine (DTIC-Dome), cis-platinum, cisplatin (PLATINOL®, PLATINOL AQ®), carboplatin (PARAPLATIN®), altretamine (HEXALEN®, etc.), antimetabolites (e.g. methotrexate (AMETHOPTERIN®, FOLEX®, MEXATE®, RHEUMATREX®), 5-fluoruracil (ADRUCIL®, EFUDEX®, FLUOROPLEX®), floxuridine, 5-fluorodeoxyuridine (FUDR), capecitabine (XELODA®), fludarabine: (FLUDARA®), cytosine arabinoside (CYTARIBINE®, CYTOSAR®, ARA-C®), 6-mercaptopurine (PURINETHOL®), 6-thioguanine (Thioguanine), gemcitabine (GEMZAR®), cladribine (LEUSTATIN®), deoxycoformycin; pentostatin (NIPENT®), etc.), antibiotics (e.g. doxorubicin (ADRIAMYCIN®, RUBEX®, DOXIL®, DAUNOXOME® liposomal preparation), daunorubicin (DAUNOMYCIN®, CERUBIDINE®), idarubicin (IDAMYCIN®), valrubicin (VALSTAR®), mitoxantrone (NOVANTRONE®), dactinomycin (ACTINOMYCIN D®, COSMEGEN®), mithramycin, plicamycin (MITHRACIN®), mitomycin C (MUTAMYCIN®), bleomycin (BLENOXANE®), procarbazine (MATULANE®, etc.), mitotic inhibitors (e.g. paclitaxel (TAXOL®)), docetaxel (TAXOTERE®), vinblatine sulfate (VELBAN®, VELSAR®, VLB®), vincristine sulfate (ONCOVIN®, VINCASAR PFS®, VINCREX®), vinorelbine sulfate (NAVELBINE®, etc.), chromatin function inhibitors (e.g., topotecan (CAMPTOSAR®), irinotecan (HYCAMTIN®), etoposide (VP-16®, VEPESID®, TOPOSAR®), teniposide (VM-26®, VUMON®, etc.), hormones and hormone inhibitors (e.g. diethylstilbesterol (STILBESTEROL®, STILPHOSTROL®), estradiol, estrogen, esterified estrogens (ESTRATAB®, MENEST®), estramustine (EMCYT®), tamoxifen (NOVALDEX®)), toremifene (FARESTON®) anastrozole (ARIMIDEX®), letrozole (FEMARA®), 17-OH-progesterone, medroxyprogesterone, megestrol acetate (MEGACE®), goserelin (ZOLADEX®), leuprolide (LEUPRON®), testosteraone, methyltestosterone, fluoxmesterone (ANDROID-F®, HALOTESTIN®), flutamide (EULEXIN®), bicalutamide (CASODEX®), nilutamide (NILANDRON®, etc.), inhibitors of synthesis (e.g., aminoglutethimide (CYTADREN®), ketoconazole (NIZORAL®, etc.)), immunomodulators (e.g., RITUXIMAB® (Rituxan), trastuzumab (HERCEPTIN®), denileukin diftitox (ONTAK®), levamisole (ERGAMISOL®), bacillus Calmette-Guerin, BCG (THERACYS®, TICE BCG), interferon alpha-2a, alpha 2b (ROFERON-A®, INTRON A®), interleukin-2, aldesleukin (PROLEUKIN®), etc.) and other agents such as 1-aspariginase (ELSPAR®, KIDROLASE®), pegaspasgase (ONCASPAR®), hydroxyurea (HYDREA®, DOXIA®), leucovorin (WELLCOVORIN®), mitotane (LYSODREN®), porfimer (PHOTOFRIN®), tretinoin (VEASNOID®), and the like.

III. Pharmaceutical Compositions.

In certain embodiments, in order to carry out the methods described herein, one or more active agents (e.g. interferon/antibody constructs described herein) are administered, e.g. to an individual diagnosed as having (or at risk for) a cancer. The active agent(s) can be administered in the “native” form or, if desired, in the form of salts, esters, amides, prodrugs, derivatives, and the like, provided the salt, ester, amide, prodrug or derivative is suitable pharmacologically, i.e., effective in the present method. Salts, esters, amides, prodrugs and other derivatives of the active agents can be prepared using standard procedures known to those skilled in the art of synthetic organic chemistry and described, for example, by March (1992) Advanced Organic Chemistry; Reactions, Mechanisms and Structure, 4th Ed. N.Y. Wiley-Interscience.

For example, acid addition salts are prepared from the free base using conventional methodology that typically involves reaction with a suitable acid. Generally, the base form of the drug is dissolved in a polar organic solvent such as methanol or ethanol and the acid is added thereto. The resulting salt either precipitates or can be brought out of solution by addition of a less polar solvent. Suitable acids for preparing acid addition salts include both organic acids, e.g., acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, malic acid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like, as well as inorganic acids, e.g., hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like. An acid addition salt may be reconverted to the free base by treatment with a suitable base. Particularly preferred acid addition salts of the active agents herein are halide salts, such as may be prepared using hydrochloric or hydrobromic acids. Conversely, preparation of basic salts of the active agents of this invention are prepared in a similar manner using a pharmaceutically acceptable base such as sodium hydroxide, potassium hydroxide, ammonium hydroxide, calcium hydroxide, trimethylamine, or the like. Particularly preferred basic salts include alkali metal salts, e.g., the sodium salt, and copper salts.

Preparation of esters typically involves functionalization of hydroxyl and/or carboxyl groups which may be present within the molecular structure of the drug. The esters are typically acyl-substituted derivatives of free alcohol groups, i.e., moieties that are derived from carboxylic acids of the formula RCOOH where R is alky, and preferably is lower alkyl. Esters can be reconverted to the free acids, if desired, by using conventional hydrogenolysis or hydrolysis procedures.

Amides and prodrugs can also be prepared using techniques known to those skilled in the art or described in the pertinent literature. For example, amides may be prepared from esters, using suitable amine reactants, or they may be prepared from an anhydride or an acid chloride by reaction with ammonia or a lower alkyl amine. Prodrugs are typically prepared by covalent attachment of a moiety that results in a compound that is therapeutically inactive until modified by an individual's metabolic system.

The active agents (e.g., constructs) described herein are useful for parenteral, topical, oral, nasal (or otherwise inhaled), rectal, or local administration, such as by aerosol or transdermally, for prophylactic and/or therapeutic treatment of one or more of the pathologies/indications described herein (e.g., atherosclerosis and/or symptoms thereof). The pharmaceutical compositions can be administered in a variety of unit dosage forms depending upon the method of administration. Suitable unit dosage forms, include, but are not limited to powders, tablets, pills, capsules, lozenges, suppositories, patches, nasal sprays, injectables, implantable sustained-release formulations, lipid complexes, etc.

In various embodiments the active agents (e.g., constructs) described herein are typically combined with a pharmaceutically acceptable carrier (excipient) to form a pharmacological composition. Pharmaceutically acceptable carriers can contain one or more physiologically acceptable compound(s) that act, for example, to stabilize the composition or to increase or decrease the absorption of the active agent(s). Physiologically acceptable compounds can include, for example, carbohydrates, such as glucose, sucrose, or dextrans, antioxidants, such as ascorbic acid or glutathione, chelating agents, low molecular weight proteins, protection and uptake enhancers such as lipids, compositions that reduce the clearance or hydrolysis of the active agents, or excipients or other stabilizers and/or buffers.

Other physiologically acceptable compounds include wetting agents, emulsifying agents, dispersing agents or preservatives that are particularly useful for preventing the growth or action of microorganisms. Various preservatives are well known and include, for example, phenol and ascorbic acid. One skilled in the art would appreciate that the choice of pharmaceutically acceptable carrier(s), including a physiologically acceptable compound depends, for example, on the route of administration of the active agent(s) and on the particular physio-chemical characteristics of the active agent(s).

The excipients are preferably sterile and generally free of undesirable matter. These compositions may be sterilized by conventional, well-known sterilization techniques.

In therapeutic applications, the constructs described herein or formulations comprising such constructs are administered to a subject, e.g., to patient suffering e.g. from a cancer, or at risk of cancer (e.g. after surgical removal of a primary tumor) in an amount sufficient to prevent and/or cure and/or or at least partially prevent or arrest the disease and/or its complications. An amount adequate to accomplish this is defined as a “therapeutically effective dose.” Amounts effective for this use will depend upon the severity of the disease and the general state of the patient's health. Single or multiple administrations of the compositions may be administered depending on the dosage and frequency as required and tolerated by the patient. In any event, the composition should provide a sufficient quantity of the active agents of the formulations of this invention to effectively treat (ameliorate one or more symptoms) the patient.

The concentration of active agent(s) can vary widely, and will be selected primarily based on fluid volumes, viscosities, body weight and the like in accordance with the particular mode of administration selected and the patient's needs. Concentrations, however, will typically be selected to provide dosages ranging from about 0.1 or 1 mg/kg/day to about 50 mg/kg/day and sometimes higher. Typical dosages range from about 3 mg/kg/day to about 3.5 mg/kg/day, preferably from about 3.5 mg/kg/day to about 7.2 mg/kg/day, more preferably from about 7.2 mg/kg/day to about 11.0 mg/kg/day, and most preferably from about 11.0 mg/kg/day to about 15.0 mg/kg/day. In certain preferred embodiments, dosages range from about 10 mg/kg/day to about 50 mg/kg/day. In certain embodiments, dosages range from about 20 mg to about 50 mg given orally twice daily. It will be appreciated that such dosages may be varied to optimize a therapeutic regimen in a particular subject or group of subjects.

In certain embodiments, the active agents (e.g., constructs described herein) are administered orally (e.g. via a tablet) or as an injectable in accordance with standard methods well known to those of skill in the art. In other preferred embodiments, the constructs may also be delivered through the skin using conventional transdermal drug delivery systems, i.e., transdermal “patches” wherein the active agent(s) are typically contained within a laminated structure that serves as a drug delivery device to be affixed to the skin. In such a structure, the drug composition is typically contained in a layer, or “reservoir,” underlying an upper backing layer. It will be appreciated that the term “reservoir” in this context refers to a quantity of “active ingredient(s)” that is ultimately available for delivery to the surface of the skin. Thus, for example, the “reservoir” may include the active ingredient(s) in an adhesive on a backing layer of the patch, or in any of a variety of different matrix formulations known to those of skill in the art. The patch may contain a single reservoir, or it may contain multiple reservoirs.

In one embodiment, the reservoir comprises a polymeric matrix of a pharmaceutically acceptable contact adhesive material that serves to affix the system to the skin during drug delivery. Examples of suitable skin contact adhesive materials include, but are not limited to, polyethylenes, polysiloxanes, polyisobutylenes, polyacrylates, polyurethanes, and the like. Alternatively, the drug-containing reservoir and skin contact adhesive are present as separate and distinct layers, with the adhesive underlying the reservoir which, in this case, may be either a polymeric matrix as described above, or it may be a liquid or hydrogel reservoir, or may take some other form. The backing layer in these laminates, which serves as the upper surface of the device, preferably functions as a primary structural element of the “patch” and provides the device with much of its flexibility. The material selected for the backing layer is preferably substantially impermeable to the active agent(s) and any other materials that are present.

In certain embodiments elevated serum half-life can be maintained by the use of sustained-release protein “packaging” systems. Such sustained release systems are well known to those of skill in the art. In one preferred embodiment, the PROLEASE® biodegradable microsphere delivery system for proteins and peptides (see, e.g., Tracy (1998) Biotechnol. Prog. 14: 108; Johnson et al. (1996), Nature Med. 2: 795; Herbert et al. (1998), Pharmaceut. Res. 15, 357) a dry powder composed of biodegradable polymeric microspheres containing the active agent in a polymer matrix that can be compounded as a dry formulation with or without other agents.

The PROLEASE® microsphere fabrication process was specifically designed to achieve a high encapsulation efficiency while maintaining integrity of the active agent. The process consists of (i) preparation of freeze-dried drug particles from bulk by spray freeze-drying the drug solution with stabilizing excipients, (ii) preparation of a drug-polymer suspension followed by sonication or homogenization to reduce the drug particle size, (iii) production of frozen drug-polymer microspheres by atomization into liquid nitrogen, (iv) extraction of the polymer solvent with ethanol, and (v) filtration and vacuum drying to produce the final dry-powder product. The resulting powder contains the solid form of the active agents, which is homogeneously and rigidly dispersed within porous polymer particles. The polymer most commonly used in the process, poly(lactide-co-glycolide) (PLG), is both biocompatible and biodegradable.

Encapsulation can be achieved at low temperatures (e.g., −40° C.). During encapsulation, the protein is maintained in the solid state in the absence of water, thus minimizing water-induced conformational mobility of the protein, preventing protein degradation reactions that include water as a reactant, and avoiding organic-aqueous interfaces where proteins may undergo denaturation. A preferred process uses solvents in which most proteins are insoluble, thus yielding high encapsulation efficiencies (e.g., greater than 95%).

In another embodiment, one or more components of the solution can be provided as a “concentrate”, e.g., in a storage container (e.g., in a premeasured volume) ready for dilution, or in a soluble capsule ready for addition to a volume of water.

The foregoing formulations and administration methods are intended to be illustrative and not limiting. It will be appreciated that, using the teaching provided herein, other suitable formulations and modes of administration can be readily devised.

IV. Kits.

In certain embodiments, kits for the treatment of a primary cancer and/or in an adjunct therapy are provided. In various embodiments the kits typically comprise a container containing a construct described herein (e.g., anti-CSPG4-IFNα, anti-CSPG4-mutIFNα, anti-CSPG4-IFN, etc.). In various embodiments the construct can be present in a pharmacologically acceptable excipient.

In addition the kits can optionally include instructional materials disclosing means of use of the chimeric moiety (e.g. to treat a cancer and/or as an adjunct therapeutic). The instructional materials may also, optionally, teach preferred dosages, counter-indications, and the like.

The kits can also include additional components to facilitate the particular application for which the kit is designed. Thus, for example, in certain embodiments, the kit can additionally contain one or more additional anti-cancer drugs (e.g., doxirubicin, vinblastine, etc.), and the like.

While the instructional materials typically comprise written or printed materials they are not limited to such. Any medium capable of storing such instructions and communicating them to an end user is contemplated by this invention. Such media include, but are not limited to electronic storage media (e.g., magnetic discs, tapes, cartridges, chips), optical media (e.g., CD ROM), and the like. Such media may include addresses to internet sites that provide such instructional materials.

EXAMPLES

The following examples are offered to illustrate, but not to limit the claimed invention.

Example 1 Anti-CSPG4 Fusions with Type I Interferon for the Treatment of Malignancy

The type 1 interferons (IFNα and IFNβ) are potent regulators of cell growth, with inhibitory effects against many human cancers (Pestka et al. (2004) Immunol. Rev. 202: 8-32; Borden et al. (2005) J. Interferon Cytokine Res. 25: 511-527; Borden et al. (2007) Nat. Rev. Drug Discov. 6: 975-690; Brassard et al. (2002) J. Leukoc. Biol. 71: 565-581; Bekisz et al. (2010) Pharmaceuticals (Basel) 3: 994-1015). Until now however, the clinical use of these agents has been limited by the inability to achieve effective concentrations of IFN at sites of tumor without causing systemic toxicity. A goal of this study is to overcome this limitation by using the tumor-targeting ability of monoclonal antibodies to carry interferons (IFNs) directly to cancer sites to mediate not only tumor destruction but also possibly to recruit the immune system to recognize and destroy residual cancer cells. It was hypothesized that antibody-IFN fusion proteins could be highly effective cancer therapeutic agents, selectively localizing IFN to sites of tumor.

Type I Interferons as Anti-Cancer Agents

Type I interferons consist of seven classes with IFNα and IFNβ being the most abundant (Pestka et al. (2004) Immunol. Rev. 202: 8-32). Type I IFNs are pleiotropic cytokines with a broad spectrum of anti-cancer activities attractive for treating melanoma and other cancers (Borden et al. (2005) J. Interferon Cytokine Res. 25: 511-527; Borden et al. (2007) Nat. Rev. Drug Discov. 6: 975-690; Brassard et al. (2002) J. Leukoc. Biol. 71: 565-581; Bekisz et al. (2010) Pharmaceuticals (Basel) 3: 994-1015). Beneficial properties of IFNα/β against cancers include, but are not limited to, direct anti-proliferative and pro-apoptotic effects (Grimley et al. (1998) Blood, 91: 3017-3027; Yanase et al. (2000) J. Interferon Cytokine Res. 20: 1121-1129; Chawla-Sarkar et al. (2003) Apoptosis 8: 237-249), blockade of autocrine growth factor loops (Heslop et al. (1990) J. Exp. Med. 172: 1729-1734), repression of c-myc oncogene expression (Einat et al. (1985) Nature, 313: 597-600), down-regulation of telomerase activity (Xu et al. (2000) Blood, 96: 4313-4318), and inhibition of angiogenesis (Borden et al. (2005) J. Interferon Cytokine Res. 25: 511-527). Favorable immunologic effects of IFNα/β for cancer treatment include activation of T cell, NK cell, and dendritic cell functions, as well as upregulation of class I MHC molecules on the tumor cell surface (Sikora et al. (2009) J. Immunol. 182(12): 7398-7407; Paquette et al. (1998) J. Leukoc. Biol. 64: 358-367; Papewalis et al. (2008) J. Immunol., 180: 1462-1470). All type I IFNs are recognized by a single shared receptor composed of two transmembrane proteins, IFNAR1 and IFNAR2. Features of IFNβ compared to IFNα include stronger receptor binding (Lamken et al. (2004) J. Mol. Biol. 341: 303-318) and more potent anti-proliferative activities (Jaitin et al. (2006) Mol. Cell Biol. 26: 1888-1897).

Recombinant IFNα (rIFNα) has clinical activity against follicular non-Hodgkin lymphoma, melanoma, multiple myeloma, chronic lymphocytic leukemia, hairy cell leukemia, chronic myelogenous leukemia, AIDS-related Kaposi sarcoma, and renal cell carcinoma (Borden et al. (2007) Nat. Rev. Drug Discov. 6: 975-690; Bekisz et al. (2010) Pharmaceuticals (Basel) 3: 994-1015; Jonasch and Haluska (2001) Oncologist 6: 34-55). However, in most of these cases the clinical effects are modest since systemic administration is dose-limited by side effects including flu-like symptoms, fatigue, nausea/anorexia, neutropenia, neuropsychiatric symptoms, and injection site reactions (Jonasch and Haluska (2001) Oncologist 6: 34-55). Also contributing to the limited clinical utility of IFNα is its short serum half-life (5 hours), and the lack of effective levels of the cytokine within tumor sites. Pharmacokinetic studies have indicated that only 0.01% of subcutaneously injected IFNα reaches the target tumor site (Suzuki et al. (2003) Gene Ther, 10: 765-773). Given these limitations, it has been difficult to achieve effective concentrations of IFNα at sites of malignant disease without causing systemic toxicity.

IFNα for Treatment of Malignant Melanoma

Recombinant interferon alpha (rIFNα) is an FDA-approved treatment for high-risk resected melanoma, a setting in which studies have shown improved survival, and is also used to treat metastatic disease (Garbe et al. (2011) Oncologist, 16(1): 5-24; Pasquali and Mocellin (2010) Curr. Med. Chem. 17(29): 3327-3336). However, while melanoma cells are very sensitive to growth inhibition to high concentrations of rIFNα in vitro (Bekisz et al. (2010) Pharmaceuticals (Basel) 3: 994-1015), these levels are not attainable by dosing with the free, soluble cytokine. Antibody-targeted delivery of IFNα/β to sites of melanoma was tested to determine if such an approach could be efficacious, and potentially associated with much less systemic toxicity.

Chondroitin Sulfate Proteoglycan 4 (CSPG4, Also Known as High Molecular Weight-Melanoma-Associated Antigen, HMW-MAA) as a Cell Surface Target on Malignant Melanoma Cells

Because of its expression in over 80% of human melanomas and its restricted distribution in normal tissues, CSPG4 has been studied as a target for the immunotherapy of melanoma (Campoli et al. (2004) Crit. Rev. Immunol. 24(4): 267-296; Campoli et al. (2010) Adv. Cancer Res. 109: 73-121). While a monoclonal antibody targeting CSPG4 can slow the growth of human melanoma cells engrafted into immunodeficient mice (Hafner et al. (2005) Int. J. Cancer, 114: 4261-4432), such antibodies have not been found to be effective in vivo against melanoma in humans (Campoli et al. (2004) Crit. Rev. Immunol. 24(4): 267-296; Campoli et al. (2010) Adv. Cancer Res. 109: 73-121).

Results

Recombinant Antibody-IFNα Fusion Proteins Targeting the CSPG4 are Efficacious Against Human Melanoma Cells In Vitro and In Vivo

Recombinant forms of the monoclonal antibody 9.2.27 that recognizes CSPG4 (Morgan et al. (1981) Hybridoma, 1(1): 27-36) were engineered into the backbone of human IgG1 (FIG. 2). These included a native form of the antibody, a fusion containing human IFNα, and a fusion containing a mutant IFNα2 (mutIFNα) that mimics IFNβ in terms of higher affinity binding to IFNAR1 (Eyal et al. (2007) J. Biol. Chem. 282(15): 11602-11611). The recombinant anti-CSPG4 antibody recognized 2 different human melanoma cell lines (SK-MEL-28 and A375) in a specific manner, showing high-level binding (FIG. 3). Both of these cell lines were sensitive to growth inhibition by free, recombinant human IFNα and IFNβ, with IFNβ being more effective, as expected (FIG. 4). The ability of the engineered IFNα fusion proteins to inhibit the in vitro growth of these cell lines was tested. As shown in FIG. 5, the native IgG1 anti-CSPG4 antibody had no effect on the growth of either cell line. The anti-CSPG4-hIFNα fusion was effective at modestly inhibiting the growth of SK-MEL-28 cells, and inhibiting A375 proliferation by 50-60%. By contrast, the anti-CSPG4-mutIFNα fusion potently inhibited the growth of both cell lines, achieving approximately 70% inhibition of SK-MEL-28 and 80% inhibition of A375. Anti-CSPG4-IFNα and anti-CSPG4-mutIFNα also inhibited the proliferation of melanoma cells with different BRAF and NRAS mutations (FIG. 6) with anti-CSPG4-mutIFNα more effective than anti-CSPG4-IFNα.

To test the ability of the fusion proteins to inhibit tumor growth in vivo, A375 cells were grown as subcutaneous xenografts in immunodeficient SCID mice (FIG. 7). After 5 days of tumor growth, mice were treated with a short course (days 5, 12, and 19) of intravenous phosphate buffered saline control, native anti-CSPG4 IgG1, anti-CSPG4-IFNα, or anti-CSPG4-mutIFNα. In mice treated with saline, tumors grew progressively, and all tumors had reached 1.4 cm in diameter before day 40. Treatment with native antibody or anti-CSPG4-IFNα appeared to slightly delay tumor growth (all tumors reaching 1.4 cm in diameter by day 44) although these differences were not statistically significant compared to saline control (p=0.073 and p=0.079, respectively). However, treatment with anti-CSPG4mutIFNα significantly delayed the growth of tumors compared to saline control (p=0.003), native antibody (p=0.0048), and anti-CSPG4-IFNα (p=0.0048). Notably, tumors did not appear to progress in mice treated with anti-CSPG4-mutIFNα until after day 19, when therapy was stopped, suggesting ongoing suppression of tumor growth in vivo. Thus, even a brief course of low-dose anti-CSPG4-mutIFNα fusion protein therapy was able to significantly delay the growth a human melanoma in vivo.

Example 2 Anti-CSPG4-Fusions with IFN Gamma (IFNγ)

A number of anti-CSPG4-Interferon gamma constructs comprising the 9.2.27 antibody were recombinantly expressed using different linkers (see Table 6). IFNγ functions as an anti-parallel dimer. Accordingly two different types of constructs were utilized. In one construct, each CH3 domain comprising the antibody had an IFNγ attached to the carboxyl terminus. In another embodiment, two interferons were attached to the CH3 domain of each antibody joined by a landar linker.

The fusion proteins with different linkers all used the anti-CSPG4 (9.2.27) described in Example 1. Their activity was analyzed against different cell lines as described below.

TABLE 6  Linkers utilized in anti-CSPG4-IFNγ constructs. Linker Amino Acid Name Sequence Nucleotide Sequence Landar LTEEQQEGGG CTTACCGAGGAGCAGCAGGAGGGC (SEQ ID NO: 71 GGCGGC (SEQ ID NO: 76) Double LTEEQQEGGG- CTTACCGAGGAGCAGCAGGAGGGC Landar* hIFNγ-TEEQQEGGG GGCGGC-hIFNgamma nt  (SEQ ID NO: 77) sequence-ACCGAGGAGCAGCAG GAGGGCGGCGGC (SEQ ID NO: 78) 1qo0E_1 LAKLKQKTEQLQDRI CTTGCTAAATTAAAACAAAAAACT AGGG GAACAATTACAAGATCGTATTGCT (SEQ ID NO: 73) GGTGGCGGC (SEQ ID NO: 79) IgG3 LELKTPLGDTTHTCP CTTGAGCTCAAAACCCCACTTGGT hinge RCPEPKSCDTPPPCPR GACACAACTCACACATGCCCACGG CPEPKSCDTPPPCPRC TGCCCAGAGCCCAAATCTTGTGAC PEPKSCDTPPPCPRCP ACACCTCCCCCGTGCCCAAGGTGC GG CCAGAGCCCAAATCTTGTGACACA (SEQ ID NO: 74) CCTCCCCCGTGCCCAAGGTGCCCA GAGCCCAAATCTTGTGACACACCT CCCCCGTGCCCAAGGTGCCCAGGC GGC (SEQ ID NO: 80) IgG1 LEPKSSDKTHTSPPSP CTTGAGCCCAAATCTTCCGACAAA hinge GG ACTCACACATCTCCACCGTCCCCA cys (SEQ ID NO: 75) GGCGGC (SEQ ID NO: 81) *Double landar = landar linker joining INF to antibody and second landar joining second IFN to first IFN. Leading “L” on linker is optional, can be introduced to permit construction of restriction site in nucleotide construct.

The amino acid sequence of components of these various constructs are shown in Table 7.

TABLE 7  Amino acid and nucleic acid sequences of linker-Interferon gamma components that are attached to the anti-CSPG4 antibody. Amino acid sequence LTEEQQEGGGQDPYVKEAENLKKYFNAGHSDVADNGTLFLGI of Landar hIFNγ: LKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKE DMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQV MAELSPAAKTGKRKRSQM (SEQ ID NO: 82) Nucleotide sequence  CTTACCGAGGAGCAGCAGGAGGGCGGCGGCCAGGATCCCT of Landar hIFNγ: ACGTGAAGGAGGCCGAGAACCTGAAGAAGTACTTCAACGC CGGCCACTCCGACGTGGCCGACAACGGCACCCTGTTCCTGG GCATCCTGAAGAACTGGAAGGAGGAGTCCGACAGGAAGAT CATGCAGTCCCAGATCGTGTCCTTCTACTTCAAGCTGTTCA AGAACTTCAAGGACGACCAGTCCATCCAGAAGTCCGTGGA GACCATCAAGGAGGACATGAACGTGAAGTTCTTCAACTCC AACAAGAAGAAGAGGGACGACTTCGAGAAGCTGACCAACT ACTCCGTGACCGACCTGAACGTGCAGAGGAAGGCCATCCA CGAGCTGATCCAGGTGATGGCCGAGCTGTCCCCCGCCGCCA AGACCGGCAAGAGGAAGAGGTCCCAGATG (SEQ ID NO: 83) Amino acid sequence LTEEQQEGGGQDPYVKEAENLKKYFNAGHSDVADNGTLFLGI of Double Landar LKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKE hIFNγ: DMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQV MAELSPAAKTGKRKRSQMTEEQQEGGGQDPYVKEAENLKKY FNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLF KNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYS VTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQM (SEQ ID NO: 84) Nucleotide sequence CTTACCGAGGAGCAGCAGGAGGGCGGCGGCCAGGACCCCT of Double Landar ACGTGAAGGAGGCCGAGAACCTGAAGAAGTACTTCAACGC hIFNγ: CGGCCACTCCGACGTGGCCGACAACGGCACCCTGTTCCTGG GCATCCTGAAGAACTGGAAGGAGGAGTCCGACAGGAAGAT CATGCAGTCCCAGATCGTGTCCTTCTACTTCAAGCTGTTCA AGAACTTCAAGGACGACCAGTCCATCCAGAAGTCCGTGGA GACCATCAAGGAGGACATGAACGTGAAGTTCTTCAACTCC AACAAGAAGAAGAGGGACGACTTCGAGAAGCTGACCAACT ACTCCGTGACCGACCTGAACGTGCAGAGGAAGGCCATCCA CGAGCTGATCCAGGTGATGGCCGAGCTGTCCCCCGCCGCCA AGACCGGCAAGAGGAAGAGGTCCCAGATGACCGAGGAGC AGCAGGAGGGCGGCGGCCAGGATCCCTACGTGAAGGAGGC CGAGAACCTGAAGAAGTACTTCAACGCCGGCCACTCCGAC GTGGCCGACAACGGCACCCTGTTCCTGGGCATCCTGAAGA ACTGGAAGGAGGAGTCCGACAGGAAGATCATGCAGTCCCA GATCGTGTCCTTCTACTTCAAGCTGTTCAAGAACTTCAAGG ACGACCAGTCCATCCAGAAGTCCGTGGAGACCATCAAGGA GGACATGAACGTGAAGTTCTTCAACTCCAACAAGAAGAAG AGGGACGACTTCGAGAAGCTGACCAACTACTCCGTGACCG ACCTGAACGTGCAGAGGAAGGCCATCCACGAGCTGATCCA GGTGATGGCCGAGCTGTCCCCCGCCGCCAAGACCGGCAAG AGGAAGAGGTCCCAGATG (SEQ ID NO: 85) Amino acid sequence LAKLKQKTEQLQDRIAGGGQDPYVKEAENLKKYFNAGHSDV of 1qo0E_1-hIFNγ: ADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQS IQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQR KAIHELIQVMAELSPAAKTGKRKRSQM (SEQ ID NO: 86) Nucleotide sequence CTTGCTAAATTAAAACAAAAAACTGAACAATTACAAGATC of 1qo0E_1-hIFNγ: GTATTGCTGGTGGCGGCCAGGATCCCTACGTGAAGGAGGC CGAGAACCTGAAGAAGTACTTCAACGCCGGCCACTCCGAC GTGGCCGACAACGGCACCCTGTTCCTGGGCATCCTGAAGA ACTGGAAGGAGGAGTCCGACAGGAAGATCATGCAGTCCCA GATCGTGTCCTTCTACTTCAAGCTGTTCAAGAACTTCAAGG ACGACCAGTCCATCCAGAAGTCCGTGGAGACCATCAAGGA GGACATGAACGTGAAGTTCTTCAACTCCAACAAGAAGAAG AGGGACGACTTCGAGAAGCTGACCAACTACTCCGTGACCG ACCTGAACGTGCAGAGGAAGGCCATCCACGAGCTGATCCA GGTGATGGCCGAGCTGTCCCCCGCCGCCAAGACCGGCAAG AGGAAGAGGTCCCAGATG (SEQ ID NO: 87) Amino acid sequence LELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCP of IgG3 hinge-hIFNγ: RCPEPKSCDTPPPCPRCPGGQDPYVKEAENLKKYFNAGHSDV ADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQS IQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQR KAIHELIQVMAELSPAAKTGKRKRSQM (SEQ ID NO: 88) Nucleotide sequence CTTGAGCTCAAAACCCCACTTGGTGACACAACTCACACATG of IgG3 hinge-hIFNγ: CCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCC CGTGCCCAAGGTGCCCAGAGCCCAAATCTTGTGACACACCT CCCCCGTGCCCAAGGTGCCCAGAGCCCAAATCTTGTGACAC ACCTCCCCCGTGCCCAAGGTGCCCAGGCGGCCAGGATCCCT ACGTGAAGGAGGCCGAGAACCTGAAGAAGTACTTCAACGC CGGCCACTCCGACGTGGCCGACAACGGCACCCTGTTCCTGG GCATCCTGAAGAACTGGAAGGAGGAGTCCGACAGGAAGAT CATGCAGTCCCAGATCGTGTCCTTCTACTTCAAGCTGTTCA AGAACTTCAAGGACGACCAGTCCATCCAGAAGTCCGTGGA GACCATCAAGGAGGACATGAACGTGAAGTTCTTCAACTCC AACAAGAAGAAGAGGGACGACTTCGAGAAGCTGACCAACT ACTCCGTGACCGACCTGAACGTGCAGAGGAAGGCCATCCA CGAGCTGATCCAGGTGATGGCCGAGCTGTCCCCCGCCGCCA AGACCGGCAAGAGGAAGAGGTCCCAGATG (SEQ ID NO: 89) Amino acid sequence LEPKSSDKTHTSPPSPGGQDPYVKEAENLKKYFNAGHSDVAD of IgG1 hinge δ NGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQ cys-hIFNγ: KSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRK AIHELIQVMAELSPAAKTGKRKRSQM (SEQ ID NO: 90) Nucleotide sequence CTTGAGCCCAAATCTTCCGACAAAACTCACACATCTCCACC of IgG1 hinge δ GTCCCCAGGCGGCCAGGATCCCTACGTGAAGGAGGCCGAG cys-hIFNγ: AACCTGAAGAAGTACTTCAACGCCGGCCACTCCGACGTGG CCGACAACGGCACCCTGTTCCTGGGCATCCTGAAGAACTGG AAGGAGGAGTCCGACAGGAAGATCATGCAGTCCCAGATCG TGTCCTTCTACTTCAAGCTGTTCAAGAACTTCAAGGACGAC CAGTCCATCCAGAAGTCCGTGGAGACCATCAAGGAGGACA TGAACGTGAAGTTCTTCAACTCCAACAAGAAGAAGAGGGA CGACTTCGAGAAGCTGACCAACTACTCCGTGACCGACCTGA ACGTGCAGAGGAAGGCCATCCACGAGCTGATCCAGGTGAT GGCCGAGCTGTCCCCCGCCGCCAAGACCGGCAAGAGGAAG AGGTCCCAGATG (SEQ ID NO: 91)

A375 myeloma cells were incubated with differing concentrations of the indicated fusion protein or with recombinant IFNγ for 4 days. The metabolic activity of the remaining cells was then measured using the MTS assay. As illustrated in FIG. 8, anti-CSPG4-fusions with IFNγ show differing activities depending on the linker used for the fusion protein.

T98 cells were incubated with differing concentrations of the indicated fusion protein or with recombinant IFNγ for 72 hours. The metabolic activity of the remaining cells was then measured using the MTS assay. As illustrated in FIG. 9, anti-CSPG4-fusions with IFNγ show differing activities against the T98 glioblastoma depending on the linker used for the fusion protein.

U87 cells were incubated with differing concentrations of the indicated fusion protein or with recombinant IFNγ for 6 days. The metabolic activity of the remaining cells was then measured using the MTS assay. As illustrated in FIG. 10, anti-CSPG4-fusions with IFNγ are more effective than IFNγ in inhibiting the growth of the U87 glioblastoma.

U373 cells were incubated with differing concentrations of the indicated fusion protein or with recombinant IFNγ for 6 days. The metabolic activity of the remaining cells was then measured using the MTS assay. As illustrated in FIG. 11, anti-CSPG4-fusions with IFNγ are more effective than IFNγ in inhibiting the growth of the U373 glioblastoma.

DBTRG-05 glioblastoma cells were incubated with differing concentrations of the indicated fusion protein or with recombinant IFNγ for 6 days. DBTRG-05 expresses little to no CSPG4. The metabolic activity of the remaining cells was then measured using the MTS assay. As illustrated in FIG. 12 anti-CSPG4-fusions with IFNγ are as effective as IFNγ in inhibiting the growth the DBTRG-05 glioblastoma.

U87, U373 and DBTRG-05 glioblastoma cells were incubated with the indicated proteins (see FIG. 13) for 24 hours. They were then detached using 2 mM EDTA, stained with anti-MHC 1, and analyzed by flow cytometry. As illustrated in FIG. 13, anti-CSPG4 IFNγ fusion proteins upregulate the expression of MHC 1 in glioblastoma cells.

OVCAR3 cells were incubated with differing concentrations of the indicated fusion protein or with recombinant IFNγ for 6 days. The metabolic activity of the remaining cells was then measured using the MTS assay. As illustrated in FIG. 14, anti-CSPG4-fusions with IFNγ show differing activities against the OVCAR3 ovarian cancer depending on the linker used for the fusion protein.

OVCAR 3 cells were incubated with the indicated proteins for 24 hours. They were then detached using 2 mM EDTA, stained with anti-MHC 1, and analyzed by flow cytometry. As illustrated in FIG. 15, anti-CSPG4 IFNγ fusion proteins upregulate the expression of MHC 1 in OVCAR 3 ovarian cells.

Without being bound to a particular theory, it appears that the activity of the construct is strongly influenced by the linker sequence. In particular, constructs using the IgG1 and IgG3 hinges as linkers provided the highest activity.

In addition to cytotoxicity examined the ability of the fusions constructs to upregulate the expression of class I MHC, one of the activities of IFNγ, was determined. In this regard, the constructs appeared to be as active as IFNγ; those data are included.

It is also believed that the activity against glioblastoma is significant since this is typically such an untreatable tumor.

It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference in their entirety for all purposes. 

What is claimed is:
 1. A chimeric construct comprising an interferon attached to a full-length antibody that binds chondroitin sulfate proteoglycan 4 (CSPG4), where: said interferon is attached to said antibody by a peptide linker comprising the amino acid sequence LEPKSSDKTHTSPPSPGG (SEQ ID NO:75); said interferon is a mature interferon alpha 2 (SEQ ID NO: 12), or a mutant mature interferon alpha 2 comprising one or more mutations selected from the group consisting of E58A, E58L, E58N, H57A, H57M, H57Y, Q61A, Q61D, and Q61S relative to mature interferon alpha 2; and said construct when contacted to a cell that expresses or overexpresses CSPG4 cell results in the killing or inhibition of growth or proliferation of said cell.
 2. The construct of claim 1, wherein said cell that expresses or overexpresses CSPG4 is a cancer cell.
 3. The construct of claim 1, wherein said interferon comprises the mutations selected from the group consisting of H57Y, E58N, and Q61S, H57M, E58L, and Q61D, H57Y, E58L, and Q61D, E58A, and Q61S, and E58A, and Q61A; wherein said mutations are relative to mature interferon alpha
 2. 4. The construct of claim 1, w herein said antibody binds to a CSPG4 at an epitope bound by one or more antibodies selected from the group consisting of 9.2.27, 225.28, and 763.74.
 5. The construct of claim 4, w herein said antibody comprises the VH and VL domain of an antibody selected from the group consisting of 9.2.27, 225.28, and 763.74.
 6. The construct of claim 1, wherein said peptide linker joins said interferon to the carboxyl terminus of the CH3 domain of said antibody.
 7. A pharmaceutical formulation comprising a construct of claim 1 in a pharmaceutically acceptable excipient.
 8. The construct of claim 1, wherein said antibody is a full-length 9.2.27 antibody.
 9. The construct of claim 8, wherein said antibody is a human IgG1.
 10. The construct of claim 1, wherein said antibody is a full-length 225.28 antibody.
 11. The construct of claim 1, wherein said antibody is a full-length 763.74 antibody. 